HPLCによるモロヘイヤ(Corchorus olitorius)及びその加工品中のストロファンチジン配糖体及びジギトキシゲニン配糖体の分析

概要

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A combination of the extraction and hydrolyzation procedures with acidified aqueous methanol was proposed to analyse strophanthidin and digitoxigenin in Corchorus olitorius (Moroheiya) and its commercial products. Plant and product samples were refluxed in methanol-0.2 mol/LHCl (2+1) at 70℃ for 2 hrs, and then stood in methanol-1.0 mol/LHCl (2+1) at room temperature for 24 hrs. A solution of the hydrolysate from well-matured seeds was directly subjected to HPLC to determine strophanthidin and digitoxigenin. For the sample from immatured seeds, Sep-pak plus Silica cartridge was used to concentrate the genins in the hydrolysate before HPLC. Since the leaves, the stems, and the products contained large amounts of contaminants, the successive purification of the hydrolysate with GPC, Sep-pak plus Silica cartridge, and ENVI-Carb Packing tube was needed to apply HPLC. The determination limits of strophanthidin and digitoxigenin in well-matured seeds, immatured seeds and others were, 10μg/g, 1μg/g and 0.02μg/g, respectively. Recoveries from the leaves, the stems, and products spiked with the genins (each 2μg/g) were 72.2-73.0% for strophanthidin and 79.6-82.6% for digitoxigenin, respectively.
日本食品化学学会の論文
1999-11-27