Globotriaosylceramide-Expressing Burkitt's Lymphoma Cells Are Committed to Early Apoptotic Status by Rhamnose-Binding Lectin from Catfish Eggs(Biochemistry)
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概要
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Silurus asotus (catfish) egg lectin (SAL) has a strong affinity to Gal α-linked carbohydrate chains of not only glycoproteins but also glycosphingolipids such as globotriaosylceramide (Gb3). SAL uniformly bound to surfaces of Gb3-expressing (Gb3^+) Burkitt's lymphoma cells, while Gb3 molecules were interspersed on the surfaces of Gb3^+ cells. After a short period of treating Raji and Daudi cells with SAL, each cell size was 10 and 25% smaller than that of untreated cells, respectively. Treatment of Gb3^+ cells with SAL caused an increase in binding of annexin V, however, neither caspase activation nor DNA fragmentation was observed after treatment with SAL for 22h. Since SAL did not induce cell death in Gb3^+ cells, SAL may function as an inducer of early apoptotic signal. We have revealed that SAL did not bind to D-threo-1-phenyl-2-decanoylamino-3-morphorino-1-propanol (D-PDMP)-treated Raji cells, and no cell shrinkage was observed in Gb3-deficient Raji cells treated with SAL, indicating that Gb3 localized in the glycosphingolipid-enriched microdomain (GEM) was involved in SAL-induced cell shrinkage through activation of voltage-gated potassium channel Kv1.3, and that the glycoprotein ligands on Gb3-deficient Raji cells treated with SAL were not included in this phenomenon. These results suggest that SAL leads the cells to early apoptotic status via binding to Gb3 existing in GEM, and that this binding is a prerequisite condition to induce early stage of apoptosis.
- 公益社団法人日本薬学会の論文
- 2009-03-01
著者
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Murayama K
Central Laboratory Of Medical Sciences Juntendo University School Of Medicine
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Murayama Kimie
朝日生命成人病研究所 循環器科
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Fujimura Tsutomu
朝日生命成人病研究所 循環器科
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Tsutomu Fujimura
Central Laboratory Of Medical Sciences Juntendo University School Of Medicine
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Taka Hikari
東京大学 大学院医学研究科循環器内科
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Taka Hikari
Central Laboratory Of Medical Sciences Juntendo University School Of Medicine
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Murayama Kimie
Central Laboratory Of Medical Sciences Juntendo University School Of Medicine
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Ogawa Y
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Nitta K
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Nitta Kazuo
Moa Health Science Foundation
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KAWANO Tasuku
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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SUGAWARA Shigeki
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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HOSONO Masahiro
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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TATSUTA Takeo
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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OGAWA Yukiko
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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FUJIMURA Tsutomu
Central Laboratory of Medical Sciences, Juntendo University School of Medicine
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NITTA Kazuo
Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University
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Nitta Kazuo
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Ogawa Yukiko
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Kawano Tasuku
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Tatsuta Takeo
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Hosono Masahiro
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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Sugawara Shigeki
Institute Of Molecular Biomembrane And Glycobiology Tohoku Pharmaceutical University
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