皮膚ノ炭水化物,特ニGlycogen及ビ蛋白糖ニ關スル實驗的研究(I)

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While the cutaneous glycogen seems to have been complately investigated on the histopathological side long ago, the chemical side of it seems to require a good deal of further study. Only a few, such as Schondorff, Folin, Trimble and Newman and a few others, have studied this subject, and their results do not agree. As for the combined sugar (protein sugar) in the blood it was studied by Figuier long ago, and by Prof. Iizuka and his school recently with success. The author, having learned from the investigations of Urbach and his co-workers, that the blood sugar and the cutaneous sugar bear a certain proportion to each other, was led to think that the combined sugar of the blood and that of the skin might similarly bear a certain proportion to each other. He therefore planned a chemical study of the combined sugar and the glycogen in the skin. The following were his findings. Part I. On the Determination of. the Glycogen and the Protein Sugar in the Skin. For the determination of the protein sugar the author employed Prof. Iizuka's and Dr. Katayama's first and second methods and for that of the glycogen he adopted the micromethods of Pfluger, Iketani, Takahata-Kume and Bierry-Gruzewska in order to make a comparative study. The results were as follows: 1) More or less glycogen and protein sugar besides the cutaneous sugar were proved in the skin. (The total reduction was found to be about 400mg% by Iizuka's method, about 500 mg% by Katayama's first method and about 600mg% by Katayama's Second method.) 2) For the determination of the protein sugar of the skin Iizuka's method seemed most suitable. 3) The most suitable concentration of sulphuric acid to destroy the skin (ca 0.25gm. in weight) at 120℃ for 30 minutes was 0.8N. 4) On hydrolysis of the skin with 0.8N sulphuric acid at 120℃ for 30 minutes, a maximum of 94% of the glycogen was inverted and a maximum of 8% of the sugar was destroyed. 5) For the determination of the cutaneous glycogen Pfluger's method seems to be most serviceable. But when the determination of the protein sugar at the same time is needed, it seems more convenient to subtract the amount of glycogen plus sugar, obtained by Iketani's method, from the total reducing substances, as this may serve to lessen the error. 6) The carbohydrate content of the skin with and without the subcutaneous fatty tissue did not differ much, Yet it was proved as usual that both the sugar and the protein sugar contents were a little higher and the glycogen content was a little lower in the pieces of skin with some subcutaneous tissue than in those without it. 7) On comparing the carbohydrate content in the parts of the skin examined, it was proved that the sugar content in the back was less than that in the other parts, while the protein sugar content in it was the highest. The glycogen content was highest in the back, less in the flank and least in the abdomen and the extremities. Part II. On the A verage Carbohydrate Content in the Skin, with Special Reference to the Glycogen and the Protein Sugar, and the Influence of Glucose on These Substances. The author measured the content of the sugar, protein sugar and glycogen in the skin of normal rabbits and a few other normal mammals before feeding for about one year. The effect of glucose on these substances was observed for two hours, mostly after the intravenous injection of the 20 or 40 per cent. solution in amounts proportional to the weight of the animals. The methods adopted for these purposes were modifications of Prof. lizuka's, Iketani's, and Hagedorn-Jensen's. In the first case, the excised parts of the shaven pelt of the test animals with no subcutaneous fatty tissue were weighed in the balance, put into a 0.8N sulphiuric acid solution, which was heated to 100℃ in advance, and hydrolyzed immediately for 30 minutes at 120℃. This was neutralized with normal NaOH solution, and desalbuminized with Patein's reagent, neutralized again with NaOH, left for 30 minutes with zinc dust and filtered. The filtrate was then tested for its reducing substances. In the second case, the excised parts of the pelt were cut into small pieces in a 3 percent solution of trichloracetic acid and left for a few hours, and filtered. The filtrate was hydrolized for 30 minutes at 120℃. The amount of the cutaneous sugar was then subtracted from the reducing substances of the hydrolized filtrate. The modification of Hagedorn-Jensen's methode was the same as that adopted by Urhach. The results obtained were as follows: 1) The average amount of the skin sugar of normally fed rabbits was 112 mg. per cent, that of the protein sugar, 194 mg. per cent and that of the glycogen, 86 mg. per cent. 2) The sugar, the protein sugar and the glycogen of the skin seemed to have no connection the climate, nor with the sex. 3) The ratio of the cutaneous sugar to the blood sugar was 102: 100 and that of the cutaneous protein sugar to the blood combined sugar was 190: 100. 4) The cutaneous sugar in a dog's tail averaged 91 mg. per cent., the inverted sugar from glycogen averaged 188 mg per cent. and the protein sugar, 233 mg. per cent. 5) The average content of the skin sugar in the pelt of a cat's flank was 90 mg. per cent., that of the inverted sugar from glycogen, 60 mg. per cent and that of the protein sugar, 224 mg. per cent. 6) In the back pelt of the guinea-pig the average content of the skin sugar was 106 mg. per cent., that of the inverted sugar from glycogen, 46 mg. per cent. and that of the protein sugar, 180 mg. per cent. 7) In the neck pelt of the goat the average content of the skin sugar was 69 mg. per cent., of protein sugar, 220 mg. per cent. and of the inverted sugar from glycogen, 90 mg. per cent. 8) When the glucose solution was injected into the vein of a rabbit's ear, the cutaneous sugar was observed to increase with the quantity of the solution injected. The glycogen, on the contrary, seemed to decrease in most cases. The coefficient of decrease seemed most marked when a 20 percent glucose solution (1 c.c. per 1 Kg.) or a 40 per cent. solution (0.5 c.c. per 1 Kg.) was injected. As for the protein sugar, it seemed to remain unchanged or to increase a little when a 20 per cent. solution (0.5-1.0 c.c. per 1 Kg.) was given. When a 40 per cent. solution (0.7-1.0 c:c. per 1 Kg.) was injected the increase was marked. 9) When a 40 per cent. glucose solution was injected intravenously the sugar, the protein sugar and the glycogen increased successively. The coefficient of increase of the glycogen, however, was not marked and it decreased later. (For the details see the tables in the original.)

著者

松本泰雄
京都府立醫科大學

皮膚ノ炭水化物,特ニGlycogen及ビ蛋白糖ニ關スル實驗的研究(I)

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