CYCLIN D1 AND CDK4 INDUCE HYPERTROPHY THROUGH PHOSPHORYLATION OF RNA POLYMERASE II C-TERMINAL DOMAIN IN NEONATAL RAT CARDIOMYOCYTES
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概要
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We have previously reported that hypertrophic stimuli induce the expression of cyclinD1 and CDK4 in cardiomyocytes, and that overexpression of cyclin D1 and CDK4 causes hypertrophic change. Recently, it is reported that pTEFb (CDK9 and cyclinT) is involved in cardiac hypertrophy through phosphorylation of the RNA polymerase Ⅱ C-terminal domain (pol Ⅱ CTD), promoting transcript elongation by Pol Ⅱ. We assessed the hypothesis that cyclinD1/CDK4 induce hypertrophy through phosphorylation of pol Ⅱ CTD in cultured neonatal rat cardiomyocytes. In this study, we examined whether the overexpression of cyclinD1/CDK4 induces hypertrophy by using[3H] leucine incorpolation assay. CyclinD1/CDK4 overexpression increased the rate of protein synthesis by 1.55 fold, compared to those in LacZ-overexpressed cells. Further, we assayed the rate of RNA synthesis by[3H]uridine incorpolation. Results showed that CyclinD1/CDK4 cells increased the rate of RNA synthesis compared to the control cells (1.57 fold).Finally, we examined whether phophorylation of pol Ⅱ CTD is involved in cyclinD1/CDK4-induced hypertrophy. DRB, a chemical antagonist of pol Ⅱ CTD phosphorylation, decreased the rate of incorporation of[3H]leucine and uridine in cyclinD1/CDK4 cells. These data altogether suggest that cyclinD1/CDK4 activates Pol Ⅱ transcription through inducing CTD phosphorylation and is involved in cardiac hypertrophy.
著者
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Isobe Mitsuaki
Tokyo Medical and Dental University
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ITOH Haruki
Department of Cardiology, Sakakibara Heart Institute
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IIMURA Hiroyuki
The Cardiovascular Institute
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Itoh Haruki
Department Of Cardiology Sakakibara Heart Institute
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Itoh Haruki
心臓血管研究所
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Itoh Haruki
聖マリアンナ医科大学 内科学循環器
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Iinuma Hiroyuki
鹿児島大学 大学院医歯学総合研究科循環器・呼吸器・代謝内科学
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Iinuma Hiroyuki
東京健生病院
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Nobori Kiyoshi
Akita University School Of Medicine
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Ito Hiroshi
Akita University Hospital
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Itoh Haruki
Int. Medicine Yokosuka Mutual Aid Hospital.
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Adachi Susumu
Department Of Cardiovascular Medicine Tokyo Medical And Dental University
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Itoh Haruki
Heart Center The Second Department Of Internal Medicine St. Marianna University School Of Medicine H
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Itoh Haruki
Department Of Internal Medicine Chiba Shakai-hoken Hospital
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Tamamori Mimi
Tokyo Medical And Dental University
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TAMAMORI-ADACHI Mimi
Tokyo Medical and Dental University
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ADACHI Susumu
Tokyo Medical and Dental University
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KITAJIMA Shigetaka
Tokyo Medical and Dental University
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Inagaki Hiroshi
Department Of Cardiovascular Medicine Tokyo Medical And Dental University
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Ito Hiroshi
Akita University School Of Medicine
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Adachi Susumu
Department Of Cardiovascular Medicine
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Tamamori Mimi
Department Of Biochemical Genetics Medical Research Instisute Graduate School Of Tokyo Medical And D
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