1003 生理的・非生理的ひずみ範囲の伸張刺激に対する骨細胞の力学応答(S03-1 細胞・生体分子のバイオメカニクス(1),S03 細胞・生体分子のバイオメカニクス)

概要

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Osteocytes inside bone matrix should play an important role in detecting local mechanical environment. The aim of this study was, therefore, to establish a relevant in vitro model for studying mechanical responses of osteocytes in three-dimensional culture. Newly designed elastic culture well consisted of 10 separated culture spaces, which realized physiological and over-physiological strain levels. After the gel-embedded MLO-Y4 cells were cultured in the culture wells, they were subjected to 24-h mechanical stretching. Viability assay indicates that significant dead cells were observed by the application of stretching over 5000 micro-strain. Beyond this threshold, the number of dead cells linearly increased with strain magnitude. MLO-Y4 cell supernatant suffered from the strain beyond the threshold showed a significant increase in TRACP activity. These experimental findings indicate that the local death of osteocytes provides an important mechanism to target bone resorption to microdamage site.
一般社団法人日本機械学会の論文
2007-09-07