747 多方向重力が骨細胞の骨髄細胞分化因子に及ぼす影響(S01-2 細胞と組織のバイオメカニクス(2)-ミクロからマクロまで-,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

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Bone loss during spaceflight or prolonged immobility has been attributed, in part, to a reduction in osteoblast number, altered gene expression, and an increase in cell death. Specially, osteocyte which is considered to be a mechanosensing cell could deeply participate in this process. To test the hypothesis that osteocyte induces osteoporotic bone loss and negatively regulated bone remodeling, we created a novel system to simulate spaceflight microgravity combining control and experimental cells within the same in vitro environment. Recently, we found that osteocyte-like cell line MLO-Y4 cells significantly induced TRACP-positive cells under microgravity. This study was to examine the role of osteocyte in bone marrow cell differentiation, especially the effect of M-CSF and RANKL secretion on bone marrow cell differentiation. MLO-Y4 was subjected to microgravity for 24 hours with and without M-CSF and RANKL inhibiter. The M-CSF and RANKL secretion in the conditioned medium was assayed by ELISA. Furthermore, supernatants of MLO-Y4 culture were collected, and then added into bone marrow cell culture. After a week of culture, TRACP activity was colorimetrically quantified. As a result, Microgravity exposed osteocytes secreted M-CSF and RANKL. Additionally, this increased TRACP activity was suppressed by inhibition M-CSF and RANKL. The data suggested that microgravity could influence anomaly osteoclastic cell differentiation by modulating M-CSF and RANKL expression in osteocytes.
一般社団法人日本機械学会の論文
2008-08-02

747 多方向重力が骨細胞の骨髄細胞分化因子に及ぼす影響(S01-2 細胞と組織のバイオメカニクス(2)-ミクロからマクロまで-,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

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