1010 細胞間ネットワークの抑制が多方向重力環境下の骨細胞に及ぼす影響(S03-2 細胞・生体分子のバイオメカニクス(2),S03 細胞・生体分子のバイオメカニクス)

概要

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Microgravity caused by spaceflight or prolonged immobility reduces bone mass. Osteocyte which is considered to be a mechanosensing cell could deeply participate in this process. Therefore, the aim of this study was to examine the role of osteocyte in bone metabolism, especially the effect of osteocytic gap junctional communication on bone marrow cell differentiation. Osteocyte-like cell line MLO-Y4 was subjected to microgravity for 24 hours with and without gap junction blocker heptanol. Supernatants of MLO-Y4 culture were collected, and then added into bone marrow cell culture. After a week of culture, both TRACP and ALP activities were colorimetrically quantified. As a result, TRACP positive cell was significantly increased by the supernatant from MLO-Y4 culture under microgravity. Additionally, this increased TRACP activity was suppressed by blocking gap junctional communication. In contrast, the supernatants had no significant effects on ALP activity. These findings indicate that alteration of osteocytic cell-to-cell communication has a potential to control osteoclastic cell differentiation.
2007-09-07