733 機械的ダメージを受けた骨細胞の骨リモデリングにおける役割(S08-2 細胞の構造とメカノバイオロジー(2),S08 細胞の構造とメカノバイオロジー)

概要

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Bone is continuously subjected to repetitive loading, which leads to microdamage even if the strain level is within physiological range. The damaged site must be sensed and promptly repaired by remodeling process ; otherwise the accumulation of microdamage results in bone fracture. The aims of this study were, therefore, to establish a relevant in vitro model for studying mechanical responses of stretched osteocytes in three-dimensional culture, and to examine the role of osteocyte in the initial phase of bone resorption. We established a loading apparatus in which osteocyte-like cell line MLO-Y4 cells were three-dimensionally cultured inside collagen gel and subjected to cyclic stretching in over-physiological strain level. Excessive stretching at 10000με damaged the gel-embedded osteocyte, resulting in significant amount of cell death. Culture medium obtained from the damaged osteocyte contained both M-CSF and RANKL, which induced significant amount of TRACP-positive cells in bone marrow culture. It suggests that the local death of osteocytes provides an important mechanism to target bone resorption to microdamaged site.
一般社団法人日本機械学会の論文
2006-09-15