1012 マイクロバブル導入による高溶存酸素環境が三次元培養した骨芽細胞の活性に与える影響(S03-2 細胞・生体分子のバイオメカニクス(2),S03 細胞・生体分子のバイオメカニクス)

概要

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Three-dimensional cell culture is widely used for challenging to biomedical tissue reconstruction. A mass of the reconstructed tissue has been limited because of difficulty in supplying oxygen deep inside the tissue. We focused on a unique characteristic of microbubbles, and therefore newly developed a compact microbubble generator for applying microbubbles into cell culture. When MC3T3-E1 osteoblastic cells were three-dimensionally cultured in collagen gel, microbubble introduction decreased cell viability. However, relative alkaline phosphatase activity was significantly enhanced by microbubbles, meaning that microbubble-induced hyperoxia has a potential to enhance osteoblastic cell activity. Application of microbubbles could be a promising method of an effective aeration to meet high oxygen demand in dense three-dimensional cell culture.
一般社団法人日本機械学会の論文
2007-09-07