Antioxidant Therapy Attenuates Diabetes-Related Impairment of Bone Marrow Stem Cells(Vascular Medicine)
スポンサーリンク
概要
- 論文の詳細を見る
Background Bone marrow cells from humans and animals with diabetes exhibit decreased angiogenic potency, thought to be related to oxidative stress, so the present study investigated if antioxidant therapy would attenuate the diabetes-related impairment. Methods and Results Diabetic mice were given antioxidant therapy, as a daily subcutaneous injection of superoxide dismutase-mimic (10mg・kg^<-1>・day^<-1>). Diabetic and healthy mice given a vehicle treatment were used as the control. After 4 weeks of treatment, bone marrow mononuclear cells (BM-MNCs) were collected for analysis and the endothelial progenitor cells in BM-MNCs were evaluated by flow cytometry. The intracellular reactive oxygen species (ROS) levels in BM-MNCs were measured using 6-carboxy-2'7'-dichlorodihydrofluorescein diacetate. Endothelial differentiation from the BM-MNCs was estimated by immunostaining with VE-cadherin 7 days after culture. BM-MNCs from the control diabetic mice had fewer Flk-1/CD34 double-positive progenitor cells and higher intracellular ROS levels, with lower potency of endothelial differentiation than BM-MNCs from the healthy mice. Antioxidant therapy decreased the intracellular ROS level in BM-MNCs from that in the diabetic mice significantly (P<0.05), but increased significantly the percentage of endothelial progenitor cells (P<0.05) and their potency of differentiation into endothelial cells (P<0.05). Conclusions Antioxidant therapy attenuated the diabetes-related impairment of BM-MNCs by reducing oxidative stress.
- 社団法人日本循環器学会の論文
- 2008-12-20
著者
-
Li Tao-sheng
Department Of Surgery And Clinical Science Yamaguchi University Graduate School Of Medicine
-
Hamano Kimikazu
Department Of Cardiac Surgery And Clinical Sciences Yamaguchi University Graduate School Of Medicine
-
Li Tao-sheng
Department Of Surgery And Clinical Science Division Of Pediatric Surgery Yamaguchi University Gradua
-
Kubo Masayuki
Department Of Surgery And Clinical Science Yamaguchi University Graduate School Of Medicine
-
Qin Shu-lan
Department Of Surgery And Clinical Science Yamaguchi University Graduate School Of Medicine
-
Ohshima Mako
Department Of Surgery And Clinical Science Yamaguchi University Graduate School Of Medicine
関連論文
- Regression of abdominal aortic aneurysms by pharmacological inhibition of c-Jun N-terminal kinase
- Impact of intraoperative transesophageal echocardiography in cardiac and thoracic aortic surgery : Experience in 1011 cases
- Direct Hemoperfusion Using Polymyxin-B Immobilized Fiber for Septic Shock After Cardiac Surgery
- 5 Real-Time Three-Dimensional Transesophageal Echo Imaging Using a Novel Miniaturized Matrix Array Transducer(Symposium 15 (SY-15) (I) New Trends in Echocardiography,Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Alterations in gap junction proteins in atrial myocardial tissue in patients with chronic atrial fibrillation
- Computed Tomographic Angiography to Evaluate the Right Gastroepiploic Artery for Coronary Artery Bypass Grafting
- Local Implantation of Autologous Bone Marrow Cells for Therapeutic Angiogenesis in Patients With Ischemic Heart Disease : Clinical Trial and Preliminary Results
- PE-190 Involvement of JNK2 in Mechanosensing and Proinflammatory Signaling during Development of Aortic Aneurysm(PE032,Peripheral Circulation/Vascular Disease (Pathophysiology, Basic) (H),Poster Session (English),The 73rd Annual Scientific Meeting of The
- PE-115 Critical Role of JNK2 for Development of Abdominal Aortic Aneurysm in Mice(Peripheral circulation/Vascular disease(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- 2 Critical Role of JNK in Chronic Inflammation of Abdominal Aortic Aneurysms(Chronic Inflammation and Cardiovascular Diseases,Roundtable Discussion 7 (RT-07) (H),Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Atheromatous Plaque in the Distal Aortic Arch Creating the Potential for Cerebral Embolism During Cardiopulmonary Bypass
- Effects of Cardioplegic Arrest and Reperfusion on Rabbit Cardiac Ryanodine Receptors
- Early Initiation of Polymyxin B-Immobilized Fiber Therapy Effective for Septic Shock after Aortic Replacement
- Successful Aortic Valve Replacement for Infective Endocarditis in a Patient with Severe Liver Cirrhosis
- Coronary Artery Bypass Grafting in the Acute Phase After Renal Transplantation : Report of a Case
- Mitral Regurgitation Secondary to Spontaneous Papillary Muscle Rupture:Report of a Case
- c-Jun N-Terminal Kinase Regulates Matrix Metalloproteinase (MMP)-9 Activity in Human Abdominal Aortic Aneurysm
- Perioperative Features of Coronary Artery Bypass Grafting in Patients Aged 75 Years or Older
- A Sewing Needle Completely Buried in the Myocardium Removed under Extracorporeal Circulation
- Right Atrial Separation Effect for Chronic Atrial Fibrillation with Atrial Septal Defect : Report of Four Cases
- OE-175 Stabilization of Extracellular Matrix Counteracts Proinflammatory Signaling and Prevents Progression of Abdominal Aortic Aneurysm(Molecular biology, vascular-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-034 Statins Reduce NF-κ B Activation and Chemokine Secretion in Human Abdominal Aortic Aneurysm Wall(Pathophysiology of Cardiovascular Disease, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-004 HMG-CoA Reductase Inhibitors Reduce Matrix Metalloproteinase-9 Activity by Multiple Mechanisms in Human Varicose Veins(Cardiovascular pharmacology, basic/clinical-2 (H) OJ1,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting
- OE-134 Inhibition of c-Jun N-terminal Kinase Causes Regression of Abdominal Aortic Aneurysm in Murine Models(Peripheral circulation-1 (H) OE23,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OE-096 c-Jun N-terminal Kinase Is Required for Development of Abdominal Aortic Aneurysm In Vivo(Molecular Biology, Vascular 1 (H) : OE12)(Oral Presentation (English))
- Functional and Structural Remodeling of Cardiomyocytes as Arrhythmogenic Substrates : Alterations in Ca^-Regulatory Proteins and Gap Junction Proteins in Atrial Fibrillation
- 7 Three-Year Follow-Up of the Safety and Feasibility of Intramyocardial Bone Marrow Mononuclear Cell Implantation in Patients with Ischemic Heart Disease(Symposium 8 (SY8) (M) : Regeneration Medicine of Cardiovascular Diseases)(Special Program)
- Experimental and Clinical Studies Investigating the Efficacy of Distal Anastomosis with Patch Plasty in Bypass Operations with Expanded Polytetrafluoroethylene Grafts
- Right Axillary Arterial Perfusion for a Ruptured Type B Aortic Dissection: Report of a Case
- An Ascending Aortic Aneurysm Caused by Giant Cell Arteritis: Report of a Case
- Modified Bentall Operation with Concomitant Total Aortic Arch Replacement for DeBakey Type I Aortic Dissection: Report of a Case
- Independent Predictive Value of the Overall Number of Metastatic N1 and N2 Stations in Lung Cancer
- 5 Autologous Bone Marrow Cells for Myocardial Repair : the Promise and Problems(States of Science and Art in Cardiovascular Medicine,Presidential Planning Program,Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Impact of collagen subtype proportions in peritoneal tissues on inguinal hernia formation in adults and infants
- Prediction of Graft Prolongation by Mixed Lymphocyte Culture Following Anti-CD4 Monoclonal Antibody Treatment Among Different Donor-Recipient Combinations
- Coronary Bypass Surgery After Renal Transplantation
- Recovery After Prolonged Cross-clamping Tepid Blood Cardioplegia : Report of a Case
- A Video - Assisted Thoracoscopic Surgical Technique for Interruption of Patent Ductus Arteriosus
- Relationship Between Respiratory Distress and Cytokine Response After Cardiopulmonary Bypass
- Klippel-Trenaunay Syndrome Associated with Splenomegaly : Report of a Case
- Transient Increase of Cytokines in the Acute Ischemic Tissue is Beneficial to Cell-Based Therapeutic Angiogenesis
- Simvastatin reduces secretion of monocyte chemoattractant proteins and matrix metalloproteinase-9 in human abdominal aortic aneurysms
- In Vitro Assessment of the Effect of Interleukin-1β on Angiogenic Potential of Bone Marrow Cells
- Adenoma of the Nipple, Focusing on the Contrast-Enhanced Magnetic Resonance Imaging Findings : Report of a Case
- Antioxidant Therapy Attenuates Diabetes-Related Impairment of Bone Marrow Stem Cells(Vascular Medicine)
- Bone marrow stem cells contribute to cardioprotection in the late phase of ischemic preconditioning
- The Detection of Chronic Heart Graft Rejection by ^P NMR Spectroscopy
- Hypoxic Preconditioning Enhances Angiogenic Potential of Bone Marrow Cells With Aging-Related Functional Impairment
- Validation of the JapanSCORE versus the logistic EuroSCORE for predicting operative mortality of cardiovascular surgery in Yamaguchi University Hospital
- Mucosa-associated lymphoid tissue (MALT) lymphoma arising in the esophagus, stomach, and lung
- Tenascin-C is expressed in abdominal aortic aneurysm tissue with an active degradation process