Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1
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概要
- 論文の詳細を見る
■Full-Length Paper■ By a grand of Research Institute for Integral Science, Kanagawa UniversityWe previously reported that Ralstonia pickettii T1, a bacterium growing onextracellular poly-3-hydroxybutyrate (PHB), have two 3-hydroxybutyrate dehydrogenases(BDH1 and BDH2). By analysis of knockout mutants of bdh1 or bdh2 using anionexchangecolumn chromatography, it was shown that a novel BDH besides BDH1 and BDH2was present in R. pickettii T1. The third BDH (BDH3) was partially purified by columnchromatography, and the enzyme had the N-terminal amino acid sequence different fromthose of BDH1 and BDH2. In Southern blotting with bdh2 as a probe, bdh3 was detectedand cloned, and the purified gene product of bdh3 expressed in Escherichia coli showedhigher specific activity than those of BDH1 and BDH2.
- 神奈川大学の論文
著者
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Saito T
National Institute Of Advanced Industrial Science And Technology (aist)
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Saito Terumi
National Institute Of Advanced Industrial Science And Technology (aist)
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Takanashi Masahiko
Laboratory of Molecular Microbiology, Department of Biological Science, Faculty of Science, Kanagawa
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Shiraki Mari
Laboratory of Molecular Microbiology, Department of Biological Science, Faculty of Science, Kanagawa
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Shiraki M
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Shiraki Mari
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Takahashi M
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Takanashi Masahiko
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Saito Takao
Department Of Chemistry National Industrial Research Institute Of Nagoya
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