Cloning and Sequencing of the Poly (3-hydroxybutyrate)(PHB) Synthase Gene from Purple Non-Sulfur Bacteria Rhodospirillum centenum and Expression of the Gene in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
A 10-kb genomic fragment was isolated from Rhodospirillum centenum by Southern-hybridization and colony-hybridization, using a probe amplified by PCR with oligo-nucleotide primers constructed from a sequence conserved in poly (3-hydro xybutyrate)(PHB) synthase genes. After subcloning of an approximately 3-kb fragment (SmaI-EcoRV) that caused the production of PHB in Escherichia coli in the presence of β-ketothiolase (phbA), an acetoacety-CoA reductase (phbB) gene from Ralstonia eutropha H16 was obtained and sequenced. This fragment contained an open reading frame (ORF) whose amino acid sequence was highly similar to the sequences of other known PHB synthase genes, especially to a synthase from Azospirillum brasilense (74% identity).
- 神奈川大学の論文
- 2006-05-25
著者
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齊藤 光實
神奈川大・理・生物
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Saito Terumi
Kanagawa Univ. Kanagawa Jpn
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Nagano Takashi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Shiraki Mari
Laboratory of Molecular Microbiology, Department of Biological Science, Faculty of Science, Kanagawa
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Suzuki Emiko
Laboratory of Molecular Microbiology, Department of Biological Sciences, Faculty of Science, Kanagaw
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Shiraki Mari
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Shiraki Mari
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Suzuki Emiko
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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