Fermentative Production of (R)-(-)-3-Hydroxybutyrate Using 3-Hydroxybutyrate Dehydrogenase Null Mutant of Ralstonia eutropha and Recombinant Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Two systems, one using an (R)-(-)-3-hydroxybutyrate dehydrogenase (BDH) null mutant of Ralstonia eutropha and the other using a recombinant Escherichia coli strain containing a synthetic poly [(R)-(-)-3-hydroxybutyrate] (PHB) operon and an extracellular PHB depolymerase gene, were used for the fermentative production of (R)-(-)-3-hydroxybutyrate (3HB). The concentration of 3HB in the culture supernatant of the mutant R. eutropha system reached about 30mM after 5d under anaerobic conditions, although it was about 4-10mM under aerobic conditions. On the other hand, the 3HB concentration in the culture supernatant of the recombinant E. coli system reached about 70mM after 4d, indicating that about 70% of the glucose added was converted to 3HB.
- 社団法人日本生物工学会の論文
- 2006-12-25
著者
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Endo Takakazu
Yokohama Technical Research Laboratory Mitsubishi Rayon Company
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Shiraki Mari
Laboratory of Molecular Microbiology, Department of Biological Science, Faculty of Science, Kanagawa
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Shiraki Mari
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Shiraki Mari
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
関連論文
- An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells
- Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1
- Biochemical and Genetic Characterization of a D(-)-3-Hydroxybutyrate Dehydrogenase from Acidovorax sp. Strain SA1
- Catalytic Triad of Intracellular Poly(3-Hydroxybutyrate) Depolymerase(PhaZ1) in Ralstonia eutropha H16
- Fermentative Production of (R)-(-)-3-Hydroxybutyrate Using 3-Hydroxybutyrate Dehydrogenase Null Mutant of Ralstonia eutropha and Recombinant Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Sequencing of the Poly (3-hydroxybutyrate)(PHB) Synthase Gene from Purple Non-Sulfur Bacteria Rhodospirillum centenum and Expression of the Gene in Escherichia coli
- Purification and Molecular Cloning of an Intracellular 3-Hydroxybutyrate-Oligomer Hydrolase from Paucimonas lemoignei(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cloning of an Intracellular D(-)-3-Hydroxybutyrate-Oligomer Hydrolase Gene from Ralstonia eutropha H16 and Identification of the Active Site Serine Residue by Site-Directed Mutagenesis
- Thiolysis of Poly(3-hydroxybutyrate) with Polyhydroxyalkanoate Synthase from Ralstonia eutropha