Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
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Two D-(-)-3-hydroxybutyrate (3HB) dehydrogenases, BDH1 and BDH2, were isolated and purified from a poly(3-hydroxybutyrate) (PHB)-degradable bacterium, Ralstonia pickettii T1. BDH1 activity increased in R. pickettii T1 cells grown on several organic acids as a carbon source but not on 3HB, whereas BDH2 activity markedly increased in the same cells grown on 3HB or PHB. To examine their biochemical properties, bdh1 and bdh2 were cloned and overexpressed in Escherichia coli, and their purified products were characterized. The kinetic parameters indicate that BDH1 is more suitable for converting acetoacetate to 3HB than BDH2, whereas BDH2 is more efficient for the reverse reaction than BDH1. Thus, R. pickettii T1 contains two BDHs with different biochemical properties and physiological roles: BDH1 for cell growth on organic acids other than 3HB and BDH2 for cell growth on 3HB or PHB.
- 社団法人日本生物工学会の論文
- 2006-06-25
著者
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Sciences Faculty Of Science Kanagawa U
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Saito Terumi
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Takanashi Masahiko
Laboratory of Molecular Microbiology, Department of Biological Science, Faculty of Science, Kanagawa
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Takanashi Masahiko
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
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Takanashi Masahiko
Laboratory Of Molecular Microbiology Department Of Biological Science Faculty Of Science Kanagawa Un
関連論文
- An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells
- Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1
- Biochemical and Genetic Characterization of a D(-)-3-Hydroxybutyrate Dehydrogenase from Acidovorax sp. Strain SA1
- Catalytic Triad of Intracellular Poly(3-Hydroxybutyrate) Depolymerase(PhaZ1) in Ralstonia eutropha H16
- Fermentative Production of (R)-(-)-3-Hydroxybutyrate Using 3-Hydroxybutyrate Dehydrogenase Null Mutant of Ralstonia eutropha and Recombinant Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Sequencing of the Poly (3-hydroxybutyrate)(PHB) Synthase Gene from Purple Non-Sulfur Bacteria Rhodospirillum centenum and Expression of the Gene in Escherichia coli
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- Cloning of an Intracellular D(-)-3-Hydroxybutyrate-Oligomer Hydrolase Gene from Ralstonia eutropha H16 and Identification of the Active Site Serine Residue by Site-Directed Mutagenesis
- Thiolysis of Poly(3-hydroxybutyrate) with Polyhydroxyalkanoate Synthase from Ralstonia eutropha