Cloning and Characterization of Twelve S-RNase Alleles in Japanese Apricot(Prunus mume Sieb. et Zucc.)

概要

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In this study, we cloned and sequenced PCR products from S-RNase alleles of 12 cultivars to determine their S-RNase genotypes or S haplotypes. We identified a novel S^<11>-RNase, which was previously misidentified as S^6-RNase. In addition, the first and second intron sizes of S^1- to S^<11>-, and S^f-RNases were presented. Since differences among the second intron sizes of S^3-, S^9-, and S^<10>-RNases, as well as those among S^5-, S^6-, and S^<11>-RNases, appeared to be very small, we developed primer sets for allele-specific PCR amplification to distinguish these S-RNase alleles. Consequently, 12 S-RNase alleles could be distinguished by PCR amplification using consensus primers for the second intron and/or the allele-specific primer sets. Using these PCR amplification, we determined S-RNase genotypes of 14 cultivars, including 'Kagajizo' (S^6S^<10>), 'Baigo' (S^6S^<10>), and 'Orihime' (S^<11>S^f), whose S-RNase genotypes have been updated. The DNA sequence information for S-RNases and the allele-specific primer sets developed in this study would be useful for future S-RNase genotyping and thus S haplotyping in Japanese apricot.
園芸学会の論文