Insulin-stimulated Fusion of GLUT4 Vesicles to Plasma Membrane is Dependent on Wortmannin-sensitive Insulin Signaling Pathway in 3T3-L1 Adipocytes
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概要
- 論文の詳細を見る
It is established that wortmannin which completely inhibits class IA PI 3-kinaseactivation abrogated the insulin-dependent translocation of GLUT4 to the plasmamembrane in adipocytes and skeletal muscle. However, it was not clear which stepswortmannin inhibited during the whole translocation process of GLUT4. We have nowdissected the each steps of the GLUT4 trafficking in 3T3-L1 adipocytes usingexogenously-expressed GLUT4 reporter in combination with plasma membrane lawnassay. We showed that 100 nM wortmannin inhibited the fusion of GLUT4 vesicles tothe plasma membrane without affecting the movement and the subsequenttethering/docking event of GLUT4 vesicles to the membrane in 3T3-L1 adipocytes.These results suggest that wortmannin-sensitive insulin signaling pathway plays acrucial role in the fusion step of GLUT4 vesicles to the plasma membrane in 3T3-L1adipocytes.
- 神戸大学の論文
著者
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KASUGA Masato
Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University
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Kasuga Masato
神戸大学 医学系研究科糖尿病代謝内分泌内科学
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Kawaguchi Takayuki
Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Kobe Universit
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Tamori Yoshikazu
Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Kobe Universit
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Yoshikawa Mari
Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Kobe Universit
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Kanda Hajime
Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Kobe Universit
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Tamori Yoshikazu
Division Of Diabetes Metabolism And Endocrinology Department Of Internal Medicine Kobe University Gr
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Tamori Yoshikazu
Division Of Diabetes And Digestive And Kidney Diseases Department Of Clinical Molecular Medicine Kob
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Kawaguchi Takayuki
Division Of Diabetes Metabolism And Endocrinology Department Of Internal Medicine Kobe University Gr
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Kasuga Masato
Division Of Diabetes And Digestive And Kidney Diseases Department Of Clinical Molecular Medicine Kob
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Kanda Hajime
Division Of Diabetes Metabolism And Endocrinology Department Of Internal Medicine Kobe University Gr
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Yoshikawa Mari
Division Of Diabetes Metabolism And Endocrinology Department Of Internal Medicine Kobe University Gr
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