Activity and Stability of α- and β-Mannosyltransferases in Candida albicans Cells Cultured at High Temperature and at Low pH(Microbiology)
スポンサーリンク
概要
- 論文の詳細を見る
We measured the activity and stability of three mannosyltransferases to ascertain the mechanisms of changes in the antigenicity and the mannan structure of Candida albicans cells cultured at high temperature (37℃) under acidic (pH 2.0) conditions in a liquid medium. The α-1,6-mannosyltransferase (α-1,6-MT) activity of the particulate-insoluble enzyme fractions prepared from C. albicans J-1012 (J-1012) cells cultured at 37℃ was retained compared to those at 27℃, whereas β-1,2-mannosyltransferase II (β-1,2-MT II) activity was detected in the 27℃ fraction but not in the 37℃ fraction. Similar results were obtained in the fraction prepared from J-1012 cells cultured at pH 2.0. The α-1,6-MT activities of fractions prepared from C. albicans NIH B-792(B-792) strain cells cultured at 37℃ were retained compared to those at 27℃, whereas β-1,2-mannosyltransferase VI-6 (β-1,2-MT VI-6) activity was detected in the fraction of B-792 cells cultured at 27℃ but not detected in the 37℃ fraction. We also found that the β-1,2-MT II and β-1,2-MT VI-6 activity of C. albicans cells was more sensitive to both high temperature and low pH compared with α-1,6-MT activity.
- 公益社団法人日本薬学会の論文
- 2008-07-01
著者
-
Okawa Yoshio
Department of Infection and Host Defense, Tohoku Pharmaceutical University
-
Okawa Yoshio
Department Of Hygienic Chemistry Tohoku Pharmaceutical University:department Of Microbiology Tohoku
-
Goto Kouji
Department of Infection and Host Defense, Tohoku Pharmaceutical University
-
Goto Kouji
Department Of Infection And Host Defense Tohoku Pharmaceutical University
関連論文
- Conformational Analysis of β-1,2-Linked Mannobiose to Mannoheptaose, Specific Antigen of Pathogenic Yeast Candida albicans
- IN VITRO CYTOLYSIS OF MM46 TUMOR CELLS BY MOUSE PHAGOCYTES ACTIVATED BY ACIDIC MANNAN FRACTION OF BAKERS' YEAST
- PROTECTIVE EFFECT OF ACIDIC MANNAN FRACTION OF BAKERS' YEAST AGAINST STAPHYLOCOCCUS AUREUS INFECTION IN MICE
- ANTITUMOR ACTIVITY OF ACIDIC MANNAN FRACTION FROM BAKERS' YEAST
- Enzymatic Synthesis of New Oligosaccharides Using Mannosyltransferases from Candida Species and Their NMR Assignments
- Adhesion of Candida albicans to HeLa Cells : Studies Using Polystyrene Beads(Microbiology)
- Antigenicity of Cell Wall Mannans of Candida albicans and Candida stellatoidea Cultured at High Temperatures in BACTEC Medium(Miscellaneous)
- Production of Interleukin-1 Activity of Kupffer Cells from Mice Treated with the Acidic Mannan Fraction of Bakers' Yeast
- Comparison of Pathogenicity of Various Candida tropicalis Strains(Microbiology)
- Comparison of Pathogenicity of Various Candida albicans and C. stellatoidea Strains(Microbiology)
- Some Properties of β-1,2-Mannosyltransferases Related to the Biosynthesis of the Acid-Labile Oligomannosyl Side Chains in Candida albicans NIH B-792 Strain Cells
- Activity and Stability of α- and β-Mannosyltransferases in Candida albicans Cells Cultured at High Temperature and at Low pH(Microbiology)