Antigenicity of Cell Wall Mannans of Candida albicans and Candida stellatoidea Cultured at High Temperatures in BACTEC Medium(Miscellaneous)
スポンサーリンク
概要
- 論文の詳細を見る
The study of the antigenicity of pathogenic Candida albicans and Candida stellatoidea cells grown in BACTEC fungal medium (BFM) is useful for clinical analysis so as accurately to diagnose candidiasis. When C. albicans NIH A-207 was grown in BFM and fetal bovine serum-added BFM at the high temperatures of 36 and 40℃, the cell density increased, with a mixture of yeast cells, pseudohyphae, and hyphae and with full hyphal development in the cultures compared with cultivation (mostly cells in yeast form) at 27℃ in both media. The mannans produced when cells were grown at these high temperatures were less reactive by enzyme-linked immunosorbent assay with factor sera 4, 5, and 6 in the commercially available kit 'Candida Check' than were the mannans obtained following growth at 27℃. Based on ^1H-nuclear magnetic resonance analysis, the mannans from cells grown at high temperatures had lost a phosphate group and a β-1,2-linked mannopyranose unit, and had increased the number of non-reducing terminal α-1,3-linked mannopyranose units. We obtained similar results for mannans produced by C. albicans J-1012, C. albicans NIH B-792, C. albicans JCM 9061, C. stellatoidea ATCC 20408, and C. stellatoidea ATCC 36232 strains cultivated in BFM at 36℃. These results suggest that both C. albicans and C. stellatoidea cells cultured at high temperatures, irrespective of the medium and shape of the cells, alter their antigenicity and chemical structure of cell wall mannans.
- 2006-08-01
著者
-
Okawa Yoshio
Department of Infection and Host Defense, Tohoku Pharmaceutical University
-
Okawa Yoshio
Department Of Infection And Host Defense Tohoku Pharmaceutical University
-
Okawa Yoshio
Department Of Hygienic Chemistry Tohoku Pharmaceutical University:department Of Microbiology Tohoku
-
Goto Kougi
Department Of Infection And Host Defense Tohoku Pharmaceutical University
関連論文
- Conformational Analysis of β-1,2-Linked Mannobiose to Mannoheptaose, Specific Antigen of Pathogenic Yeast Candida albicans
- IN VITRO CYTOLYSIS OF MM46 TUMOR CELLS BY MOUSE PHAGOCYTES ACTIVATED BY ACIDIC MANNAN FRACTION OF BAKERS' YEAST
- PROTECTIVE EFFECT OF ACIDIC MANNAN FRACTION OF BAKERS' YEAST AGAINST STAPHYLOCOCCUS AUREUS INFECTION IN MICE
- ANTITUMOR ACTIVITY OF ACIDIC MANNAN FRACTION FROM BAKERS' YEAST
- Enzymatic Synthesis of New Oligosaccharides Using Mannosyltransferases from Candida Species and Their NMR Assignments
- Adhesion of Candida albicans to HeLa Cells : Studies Using Polystyrene Beads(Microbiology)
- Antigenicity of Cell Wall Mannans of Candida albicans and Candida stellatoidea Cultured at High Temperatures in BACTEC Medium(Miscellaneous)
- Production of Interleukin-1 Activity of Kupffer Cells from Mice Treated with the Acidic Mannan Fraction of Bakers' Yeast
- Comparison of Pathogenicity of Various Candida tropicalis Strains(Microbiology)
- Comparison of Pathogenicity of Various Candida albicans and C. stellatoidea Strains(Microbiology)
- Some Properties of β-1,2-Mannosyltransferases Related to the Biosynthesis of the Acid-Labile Oligomannosyl Side Chains in Candida albicans NIH B-792 Strain Cells
- Activity and Stability of α- and β-Mannosyltransferases in Candida albicans Cells Cultured at High Temperature and at Low pH(Microbiology)