59(P-29) 繊毛虫Climacostomum virensの自己防御機能(ポスター発表の部)

概要

論文の詳細を見る
The heterotrich ciliates Stentor coeruleus and Blepharisma japonicum have pigment granules which contain the blue pigment stentorin and the red pigment blepharismin, respectively. We found that the pigment granules are extrusomes, extrusive organellas in protists, and that the pigments function for defense against the predatory ciliate Dileptus margaritifer. Climacostomum virens, another heterotrich ciliate, is colorless, and has cortical vesicles which are morphologically similar to the pigment granules in the red species of Blepharisma. The retreating behavior of D. margaritifer observed when it attacks C. virens suggested that C. virens also has a toxin against the predator. We already reported isolation, characterization and synthesis of climacostol (1) which was a potent toxin of C. virens. When S. coeruleus and B. japonicum are mixed with SMB (Synthetic Medium for Blepharisma) at 0℃ (cold shock), they discharge pigment granules as extrusomes in a similar way as a response to the attack by the predator. The cells of C. virens were treated by cold shock to discharge the extrusomes to the medium, which showed a lethal toxicity to the predatory ciliates. Climacostol was found to be truly present in the medium by the analysis of the content using HPLC and LC/MS analysis. We concluded climacostol function as a self-defense against the predatory ciliates. We also found that the contents consisted of congeners of climacostol, 2 and 3. Their structures were assumed by the ^1H NMR spectra and ESI-LC/MS/MS analyses to be 5-undeca-2,5-dienyl-benzene-1,3-diol and (Z,Z,Z)-5-undeca-2,5,8-trienyl-benzene-1,3-diol, respectively.
天然有機化合物討論会の論文
2002-09-01

59(P-29) 繊毛虫Climacostomum virensの自己防御機能(ポスター発表の部)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク