47 植物防御系においてペルオキシダーゼが関与する化学的防御機構の解析(口頭発表の部)
スポンサーリンク
概要
- 論文の詳細を見る
Plants induce varied resistance-response toward microbial challenge. In order to establish an infection, pathogenic fungi must break such defense barriers as lignin, phytoalexins, and hydrolytic enzymes. At the early stage of infection, POD (peroxidase) activity is enhanced dramatically. POD and lignin accumulates at the infected site and then protects from further fungal invasion. The suppression of lignin deposition at the site might be of advantage to susceptible pathogenic fungi. We, therefore, conducted a comprehensive screening to obtain POD inhibitors from fungal cultures and a potent radical scavenger named ST-1 was isolated from a fungus, and its chemical structure was elucidated to be 1,3-dihydro-4,5,6-trihydroxy-1,3-dimethoxy-7-methylisobenzofuran by a combination of chemical modification and spectroscopic techniques. ST-1 effectively scavenged oxygen radicals and also suppressed the phytoalexin accumulation in kidney bean. These results suggested that such a mechanism can be enumerated as one of significant defense systems intervening plants and microbes. On the other hand, the POD, activated upon pathogenic challenge, could convert common plant phenolics into bioactive compounds which interfered with pathogen and insect attacks. This idea prompted us to examine the possibility. Guaiacol and other phenols were chosen as model substrates for the enzyme reaction system, and several products with novel structures were confirmed to have unique biological activities. This paper deals with the chemical structures of a novel radical scavenger (ST-1) and the POD products with anti-microbial activities and phytotoxicities.
- 天然有機化合物討論会の論文
- 1993-09-10
著者
関連論文
- 2Ep23 光受容体フィトクロムAを非光合成植物由来のものに置換したシロイヌナズナの光応答(植物細胞工学・組織培養・育種工学,一般講演)
- 3A-AM5 1細胞分析に資するレーザーテクノロジー(ライフサーベイヤー構築をめざしたナノバイオとコンビバイオの融合の新しい展開,シンポジウム)
- 438 酵母人工染色体のデリバリーを目的としたDNA含有ビーズ(バイオビーズ)の開発(遺伝子工学,一般講演)
- 2141 巨大 DNA 分子のデリバリーを目的としたバイオビーズの開発
- 504 DNA含有ヒーズ(バイオビーズ)による植物細胞の形質転換
- 503 巨大DNA分子のデリバリーを目的としたバイオビーズの開発
- 73.リグナン類の高感度微量分析系の確立ならびにレンギョウ中のリグナン含量と光環境の相関解析(口頭発表)
- 3S12a01 エンバク感染応答の1細胞分析(非侵襲シングルセル解析-ライフサーベイヤーのめざす生物科学-,シンポジウム)
- 68.寄生植物ヤセウツボ種子の発芽過程における特徴的な窒素代謝(口頭発表)
- 3Aa13 非光合成寄生植物ヤセウツボ由来フィトクロムAの機能解析(生物化学工学,植物,一般講演)