29 Avermectin生合成の分子遺伝学的研究(口頭発表の部)

概要

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The anthelmintic antibiotic avermectin complex produced by Streptomyces avermitilis is a family of eight closely related components. Our interests are focused breeding mutants which produce new components and selective producer of a useful component(s) alone. To resolve the above subjects, we investigated the biosynthesis of avermectin by genetic and molecular genetic approaches. Several kinds of biosynthetically blocked mutants and one multiple-blocked mutant were isolated. The latter mutant produced four new components (I, II, III and IV). All mutations of blocked mutants tested were mapped as a cluster on the chromosome. This was also supported by the result of cloning of genes for avermectin biosynthesis. Designing the selective producer which produces a useful component(s) alone was performed by in vivo recombination. One of recombinant strains produced only two components of avermectins. In addition, a toxic compound oligomycin produced by S. avermitilis is difficult to be separated from avermectin components then S. avermitilis which did not produce oligomycin were obtained by disruption of the oligomycin biosynthetic gene by transposon(Tn4560) insertion mutagenesis.
天然有機化合物討論会の論文
1992-09-10