93(PB3-4) いもち病菌の代謝産物の菌株間比較と新代謝産物Pyrichalasin Hの構造(ポスター発表の部)

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The metabolites produced by 69 strains belonging to the genus Pyricularia were compared by TLC and HPLC. Each strain was cultured in a soy sauce-sucrose medium. From the ethyl acetate extracts of their culture filtrates the following compounds were identified based on their spectroscopic data: pyriculol (1) and its dihyro derivative (3), pyriculariol (2), tenuazonic acid (5), p-hydroxy-phenylethyl alcohol (6), 6-hydroxymellein (7), 4,8-dihydroxytetralone (8), and 3,4-dihydro-3,4,8-trihydroxy-1(2H)-naphtalenone (9). The structure of a new metabolite, dihydropyriculariol (4), was established and N-acetyltyramine (10) was first identified as a metabolite of this fungal group in this study. During this studies, a new very potent phytotoxic metabolite, named pyrichalasin H (11), was isolated from P. grisea IFO 7287, and its structure was determined by spectroscopic comparisons with a known fungal metabolite, cytochalasin H (12). Pyrichalasin H was found to induce characteristic curling of the shoot of rice seedlings when treated at 1ppm and at higher concentration causing strong growth inhibition. Pyrichalasin H also showed cytotoxic activity to KB cell in vitro (IC_<50> 0.12ug/ml).
天然有機化合物討論会の論文
1987-07-25