Determination of Bactericidal Activity of Antibacterial Monomer MDPB by a Viability Staining Method
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概要
- 論文の詳細を見る
In this study, the bactericidal activity of antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) against Streptococcus mutans was tested by a rapid method for monitoring viability. To S. mutans culture containing fluorescence staining solution that distinguishes live from dead cells, MDPB was added at a concentration of 250, 100, 50, or 10μg/ml. Bacterial cells were observed by fluorescence microscopy and the percentage of dead cells was calculated. After 10, 20, or 30 minutes' contact with MDPB, the live/dead ratio was measured by fluorometry and viable counts (CFU) determined by the conventional plating method. Viability staining revealed that MDPB exhibited significant bactericidal effects at 50μg/ml or greater (ANOVA, Fisher's PLSD test), and complete killing of the cells at 250μg/ml of MDPB was demonstrated in conjunction with a plating method. The staining method thus provided a sensitive means to determine loss of viability, and indicated the strong killing effects of MDPB on S. mutans.
- 日本歯科理工学会の論文
著者
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IMAZATO Satoshi
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
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MCCABE John
Dental Materials Science Unit, School of Dental Sciences, University of Newcastle upon Tyne
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Mccabe John
Dental Materials Science Unit School Of Dental Sciences University Of Newcastle Upon Tyne
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MAEDA Nobuko
Department of Bacteriology, School of Dental Medicine, Tsurumi University
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Imazato Satoshi
Department Of Restorative Dentistry And Endodontology Osaka University Graduate School Of Dentistry
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MOMOI Yasuko
Department of Operative Dentistry, Tsurumi University School of Dental Medicine
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Russell Roy
Department Of Oral Biology University Of Newcastle Upon Tyne
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OHMORI Kaoru
Department of Operative Dentistry, Tsurumi University School of Dental Medicine
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Ohmori Kaoru
Department Of Operative Dentistry Tsurumi University School Of Dental Medicine
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Imazato Satoshi
Department Of Arts And Sciences Osaka University Of Education
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Momoi Yasuko
Department Of Operative Dentistry School Of Dental Medicine Tsurumi University
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Ohmori Kaoru
Tsurumi Univ. School Of Dental Medicine Yokohama Jpn
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