Changes in Levels and Rates of Biosynthesis of Amino Acids during the Growth of Catharanthus roseus Cells in Batch Suspension Culture

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Changes in levels of individual amino acids and rates of the biosynthesis of these amino acids from sucrose were determined at various stages of growth of Catharanthus roseus in suspension culture. The levels of most of the free amino acids decreased gradually after the cells were transferred to fresh culture medium, while levels of some amino acids, i.e. glutamine, glutamic acid, aspertic acid and phenylalanine increased transiently during the lag or early logarithmic phase of cell growth. The levels of most amino acids increased again at stationary phase. In contrast, levels of protein amino acids increased during cell proliferation and decreased during stationary phase. The labelled carbon atoms from [U-^<14>C] sucrose were incorporated into glutamic acid, glutamine, glycine, phenylalaine, serine, arginine, aspartic acid, threonine, valine, isoleucine, leucine and lysine. Except for its incorporation into some free amino acids, such as glutamic acid and glutamine, most of the radioactivity was found in the protein fraction. The rate of biosynthesis of most amino acids increased during cell proliferation and decreased during the stationary phase.
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