Nucleotide Pools and Purine Metabolism in Tissue Cultures of Wild-Type Datura innoxia and Adenine-Requiring Auxotroph

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Cells of the auxotrophic mutant, Ad1, of Datura innoxia required adenine, adenosine, or inosine for their growth on solid agar medium which contained Murashige-Skoog salts, 2,4-dichlorophenoxyacetic acid, and sucrose. Thirteen purine and pyrimidine nucleotides in extracts of wild-type and Ad1 cells were separated and quantified by HPLC. Levels of ADP-glucose and UMP were significantly higher in Ad1 than in wild-type cells, but those of other nucleotides was found when Ad1 cells were transferred to fresh medium without adenine. The rate of the biosynthesis de novo of purines, as estimated from the rate of incorporation of ^<14>C from [2-^<14>C]-glycine and [^<14>C]formate into adenine nucleotides, was reduced in Ad1 cells to 21 and 13% of the wild-type rate, respectively. The activities involved in the salvage of adenine and adenosine in Ad1 cells were similar to those in wild-type cells. Ad1 cells had the capability to convert adenine to guanine nucleotides and guanine to adenine nucleotides.
日本植物生理学会の論文