EMIT法によるミコフェノール酸血中濃度測定とHPLC法との比較による血中濃度評価

概要

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EMIT, a homogeneous enzyme immunoassay for the quantitative analysis of mycophenolic acid (MPA) in human plasma, was evaluated in comparison with high-performance liquid chromatography (HPLC). For EMIT, coefficients of variation (CV) for within-run measurements (1〜12μg/mL, n=20) varied from 4.07% to 5.16% and CVs for between-day measurements (1〜12μg/mL, n=20) ranged from 2.23 % to 5.63 %. The lower limit of detection was 0.20μg/mL. When MPA plasma concentrations were simultaneously measured using EMIT and HPLC, there was a good linear relationship between the 2 assay methods showing a high degree of correlation : EMIT=1.13×HPLC+0.164 ; r^2=0.967, 51 points in 16 liver transplant recipients, EMIT=1.10×HPLC+0.305 ; r^2=0.948, 55 points in 15 renal transplant recipients and EMIT=1.06×HPLC-0.014 ; r^2=0.887, 300 points in 8 islet transplant recipients. These results indicate that EMIT is a convenient means of measuring MPA in plasma.
日本医療薬学会の論文
2007-09-10