キャピラリー電気泳動法による生体試料中の過塩素酸塩の定量

概要

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A rapid capillary electrophoresis method was developed to detect perchlorate in tissues of male rats after the administration of perchlorate. Supernatants of ethanol-precipitated rat fluids were evaporated to dryness, and silver acetate solution was added. Precipitated silver chlorides were separated by centrifugation and supernatants were injected into a capillary electrophoresis system coupled with UV detection. The separation and quantification of perchlorate was achieved using Agilent inorganic anion buffer as the carrier electrolyte, electrokinetic injection and indirect detection at 200 nm. The lower detection limits for perchlorate in fluids and tissues of rats were 0.01 μg/ml and 0.25 mg/kg, respectively. Perchlorate in biological samples was determined for the first time by capillary electrophoresis already described.
2007-08-05