Analysis of Azotobacter vinelandii Nitrogenase Reaction by Monoclonal Antibodies(Biological Chemistry)
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概要
- 論文の詳細を見る
Nitrogenase catalyzes not only the reduction of N_2 to NH_3 but also the reduction of C_2H_2 to C_2H_4 and H^+ ion to H_2 gas, etc. The detailed mechanism of the nitrogenase reaction is not clear. We have prepared monoclonal antibodies against Component I nitrogenase of A. vinelandii and examined the effects of antibodies on the nitrogenase reactions. A monoclonal antibody designated MA-1 inhibited C_2H_2 reduction activity strongly but did not inhibit H_2 evolution activity. MA-2, on the contrary, inhibited only H_2 evolution activity. MA-8 inhibited both C_2H_2 reduction and H_2 evolution activity to the same extent.
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
-
Abe Naoto
Department Of Agricultural Chemistry The University Of Tokyo
-
Onodera K
Univ. Tokyo Tokyo Jpn
-
Onodera Kazukiyo
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Maruyama Yoshiharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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