Formation of Lipid Oligosaccharides in a Tunicamycin Resistant Mutant of Chinese Hamster Ovary Cells
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概要
- 論文の詳細を見る
The synthesis of lipid oligosaccharide intermediates of a tunicamycin resistant mutant of Chinese hamster ovary cells was investigated both in vivo and in vitro. It was shown that tunicamycin resistant mutant cells had a significantly lower rate of synthesis of lipid oligosaccharide in vivo and their pool of lipid oligosaccharide was smaller. The formation of lipid oligosaccharides in vitro using microsomes, however, did not show any defect in the enzymes which were involved in this reaction. The rate of transfer of [3H]glucose from UDP-[3H]glucose into oligosaccharide of lipid intermediates was the same in wild type and mutant cells while that into glycoproteins was higher in mutant cells than in wild type cells.
- 社団法人 日本農芸化学会の論文
著者
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Onodera Kazukiyo
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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SEHARA-FUJISAWA Atsuko
Institute for Virus Research, Kyoto University
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MASUDA Liria
Department of Agricultural Chemistry, The University of Tokyo
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- Formation of Lipid Oligosaccharides in a Tunicamycin Resistant Mutant of Chinese Hamster Ovary Cells