Involvement of Lectins in Rhizobium-Pea Recognition

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Hapten-inhibition studies showed that 3-O-methyl-D-glucose and methyl-α-D-mannopyranoside, which are strong sugar haptens of hemagglutination by pea seed lectins, inhibited (a) binding of pea seed lectins with Rhizobium leguminosarun J357 cells, (b) the precipitin reaction of pea seed lectins with a capsular polysaccharide from J357 cells and (c) adsorption of J357 cells to a pea root. When the capsular polysaccharide was absorbed by the lectins or oxidized by periodate to remove the precipitin reactivity with the lectin, the inhibitory activity of the capsular polysaccharide towards the adsorption disappeared. Lectins, which were isolated from the bathing solution of pea roots in acid buffer (pH 2.1), were similar to the seed lectins in sugar-binding specificity. The possible existence of lectins on pea root hair surface was shown by the indirect immuno-fluorescent antibody technique in combination with the biotin-avidin system. These results suggest that host recognition in Rhizobium-pea symbiosis is based on the interaction between rhizobial cells and host lectins.
日本植物生理学会の論文