Ca-enzyme Complex of Halophilic Nuclease H of Halophilic Micrococcus varians subsp. halophilus for 5'-Nucleotide Production by RNA Degradation(Microbiology & Fermentation Industry)

概要

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A new type of immobilized halophilic nuclease H, Ca-en/yme complex, from a moderate halophile, Micrococcus varians subsp. halophilm was demonstrated. The 4-day cultivation of the bacterium in 3M NaCl CM medium at 25℃ was favorable for our purpose, producing less 5'-nucleotidase, but flocculation of the cells grown at 25℃ was poorer than that of the cells grown at 30℃. On the other hand, almost all of the nuclease H was precipitated to form Ca-enzyme complex by addition of 70 mM Ca^<2+> to the supernatant of the broth. The nuclease H activity of the Ca-enzyme complex was increased by preferential adsorption of exogenous nuclease H. A column bioreactor packed with this enhanced Ca-enzyme complex was used for 5 -mononucleotide production by continuous RNA degradation.
社団法人日本農芸化学会の論文
1990-10-23