A HPLC Method for Simultaneous Analysis of NAD(P)^+ and NAD(P)H : Its Application to the Study of Spinach Ferredoxin: NADP^+ Reductase-catalyzed Transhydrogenation(Biological Chemistry)
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概要
- 論文の詳細を見る
A new HPLC system for simultaneous analysis of NAD^+, NADH, NADP^+, and NADPH was developed and used to measure the transhydrogenase activity of spinach ferredoxin-NADP^+ reductase (EC 1.18.1.2, FNR). The system is based on a reverse-phase HPLC with isocratic elution on an ODS column (4.6×50mm). The four nucleotides were completely separated by developing the column with 0.15 M sodium phosphate/citrate buffer (pH 6.8) containing 1mM EDTA at 40℃ with a flow rate of 1 ml/min. The four nucleotides can be simultaneously assayed within 13 min by monitoring the effluents with a UV detector. It was also indicated that the transhydrogenase activity of spinach FNR can be advantageously assayed by measuring the nucleotides by this method.
- 社団法人日本農芸化学会の論文
- 1989-06-23
著者
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Yamasaki Nobuyuki
Institute Of Biophysics Faculty Of Agriculture Kyushu University
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Yamasaki Nobuyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Aso Yoichi
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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GOTOH Shoji
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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Yamasaki N
Kyushu Univ. Fukuoka Jpn
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Aso Yoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Aso Y
Kyushu Univ. Fukuoka Jpn
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Gotoh Shoji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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