Biochemical Aspects of 2-Keto-L-gulonate Accumulation from 2,5-Diketo-D-gluconate by Corynebacterium sp. and Its Mutants(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• Corynebacterium sp. SHS 0007 accumulated 2-keto-L-gulonate and 2-keto-D-gluconate simultaneously with 2,5-diketo-D-gluconate utilization. This strain, however, possibly metabolized 2,5-diket-D-gluconate through two pathways leading to D-gluconate as a common intermediate: via 2-keto-D-gluconate, and via 2-keto-L-gulonate, L-idonate and 5-keto-D-gluconate. A polysaccharide-nagative, 2-keto-L-gulonate-negative and 5-keto-D-gluconate-negative mutant produced only calcium 2-keto-L-gulonate from calcium 2,5-diketo-D-gluconate, in a 90.5mol% yield. The addition of a hydrogen donor such as D-glucose was essential for its production. This mutant possessed the direct oxidation route of D-glucose to D-gluconate, the pentose cycle pathway and a possible Embden-Meyerhof-Parnas pathwey, indicating that D-glucose was metabolized through these three pathways and provided NADPH for the reduction of 2,5-diketo-D-gluconate.

• 社団法人日本農芸化学会の論文
• 1987-11-23

著者

• Yagi Shigeo

  Bioprocess Program Process R&d Laboratories Shionogi & Co.ltd.

• Yagi Shigeo

  Biotechnology Development Department Of Production Shionogi & Co. Ltd.

• Mitsushima K

  Bioprocess Program Process R&d Laboratories Shionogi & Co.ltd.

• Sonoyama T

  Bioprocess Research & Development Manufacturing Division Shionogi & Co. Ltd.

• Sonoyama Takayasu

  Biotechnology Development Department Of Production Shionogi & Co. Ltd.

• KAGEYAMA BUNJI

  Bioprocess Research and Development, Production Department, Shionogi and Co., Ltd.,

• KAGEYAMA Bunji

  Biotechnology Development, Department of Production, Shionogi & Co., Ltd.

• MITSUSHIMA Kenji

  Biotechnology Development, Department of Production, Shionogi and Co., Ltd.

• Kageyama Bunji

  Bioprocess Research And Development Production Department Shionogi And Co. Ltd.

関連論文

• High-Level Expression, Purification, and Some Properties of a Recombinant Cephalosporin-C Deacetylase
• Purification and Properties of 5-Keto-D-Fructose Reductase from a Mutant Strain Derived from Corynebacterium sp.
• trans-3-Methylthioacrylamide, a New Metabolic Product from Methionine by Streptomyces(Microbiology & Fermentation Industry)
• Purification and Properties of Two 2,5-Diketo-D-Gluconate Reductases from a Mutant Strain Derived from Corynebacterium sp.
• Purification, Characterization and Partial Amino Acid Sequences of a Novel Cephalosporin-C Deacetylase from Bacillus subtilis
• A Novel Method of Immobilizing Penicillin Acylase on Basic Anion Exchange Resin with Hyxamethylene Diisocyanate and p-Hedroxybenzaldehyde
• Stimulative Effect of Nitrate on the Production of 2-Keto-L-gulonate from 2,5-Diketo-D-gluconate by a Mutant Strain Derived from Corynebacterium sp.(Microbiology & Fermentation Industry)
• Facultatively Anaerobic Bacteria Showing High Productivities of 2,5-Diketo-D-gluconate from D-Glucose(Microbiology & Fermentation Industry)
• Biochemical Aspects of 2-Keto-L-gulonate Accumulation from 2,5-Diketo-D-gluconate by Corynebacterium sp. and Its Mutants(Microbiology & Fermentation Industry)
• Distribution of Microorganisms Capable of Reducing 2, 5-Diketo-D-gluconate to 2-Keto-L-gulonate

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