Purification and Properties of Two 2,5-Diketo-D-Gluconate Reductases from a Mutant Strain Derived from Corynebacterium sp.

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概要

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• Two 2,5-diketo-D-gluconate reductases, I and II, were purified respectively 918-fold and 28-fold from a mutant strain derived from Corynebacterium sp. SHS 0007. The enzymes appeared to be homogeneous on polyacrylamide gel electrophoresis. Both reductases converted 2,5-diketo-D-gluconate to 2-keto-L-gulonate in the presence of NADPH and seemed to be active only for reduction. The molecular weights of reductases I and II were estimated to be 29,000 and 34,000,respectively; and both were monomeric. Their isoelectric points were respectively pH 4.3 and pH 4.1. The optimum pH was 6.0 to 7.0 for reductase I, and 6.0 to 7.5 for reductase II. The K_m values (pH 7.0,30℃) of reductase I for 2,5-diketo-D-gluconate and for NADPH were 1.8 mM and 12 μM, respectively; and the corresponding values of reductase II were 13.5 mM and 13 μM. Both reductases converted 5-keto-D-fructose to L-sorbose in the presence of NADPH.

• 公益社団法人日本生物工学会の論文
• 1987-06-25

著者

• Kobayashi Kobei

  Fermentation Development Department Of Production Shionogi And Co. Ltd.

• Sonoyama T

  Bioprocess Research & Development Manufacturing Division Shionogi & Co. Ltd.

• SONOYAMA TAKAYASU

  Fermentation Development, Department of Production, Shionogi and Co., Ltd.,

関連論文

• Purification and Properties of Two 2,5-Diketo-D-Gluconate Reductases from a Mutant Strain Derived from Corynebacterium sp.
• Purification, Characterization and Partial Amino Acid Sequences of a Novel Cephalosporin-C Deacetylase from Bacillus subtilis
• A Novel Method of Immobilizing Penicillin Acylase on Basic Anion Exchange Resin with Hyxamethylene Diisocyanate and p-Hedroxybenzaldehyde
• Stimulative Effect of Nitrate on the Production of 2-Keto-L-gulonate from 2,5-Diketo-D-gluconate by a Mutant Strain Derived from Corynebacterium sp.(Microbiology & Fermentation Industry)
• Facultatively Anaerobic Bacteria Showing High Productivities of 2,5-Diketo-D-gluconate from D-Glucose(Microbiology & Fermentation Industry)
• Biochemical Aspects of 2-Keto-L-gulonate Accumulation from 2,5-Diketo-D-gluconate by Corynebacterium sp. and Its Mutants(Microbiology & Fermentation Industry)

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