A Novel Method of Immobilizing Penicillin Acylase on Basic Anion Exchange Resin with Hyxamethylene Diisocyanate and p-Hedroxybenzaldehyde
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概要
- 論文の詳細を見る
Penicillin acylase from Bacillus megaterium was immobilized to the functional groups of the basic anion exchange resin Diaion CR-20 using hexamethylene diisocyanate and p-hydroxybenzaldehyde as the coupling reagents. A specific activity of 135U/g resin was obtained with an activity yield of 45%. The addition of 0.3mM Ca^<2+> improved the stability of the immobilized enzyme, with its remaining activity in a recycling system at the 300th time being about 70% of the initial value.
- 公益社団法人日本生物工学会の論文
- 1992-12-25
著者
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Yagi Shigeo
Bioprocess Program Process R&d Laboratories Shionogi & Co.ltd.
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Sonoyama T
Bioprocess Research & Development Manufacturing Division Shionogi & Co. Ltd.
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SONOYAMA TAKAYASU
Bioprocess Research & Development, Manufacturing Division, Shionogi & Co. Ltd.,
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KOBAYASHI KOBEE
Bioprocess Research and Development, Production Department, Shionogi and Co., Ltd.,
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KAGEYAMA BUNJI
Bioprocess Research and Development, Production Department, Shionogi and Co., Ltd.,
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Kageyama Bunji
Bioprocess Research And Development Production Department Shionogi And Co. Ltd.
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Kobayashi Kobee
Bioprocess Research And Development Production Department Shionogi And Co. Ltd.
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- Purification, Characterization and Partial Amino Acid Sequences of a Novel Cephalosporin-C Deacetylase from Bacillus subtilis
- A Novel Method of Immobilizing Penicillin Acylase on Basic Anion Exchange Resin with Hyxamethylene Diisocyanate and p-Hedroxybenzaldehyde
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