Amphipathic alpha-helix mediates the heterodimerization of soluble guanylyl cyclase.
スポンサーリンク
概要
- 論文の詳細を見る
Soluble guanylyl cyclase (soluble GC) is an enzyme consisting of α and β subunits and catalyzes the conversion of GTP to cGMP. The formation of the heterodimer is essential for the activity of soluble GC. Each subunit of soluble GC has been shown to comprize three functionally different parts: a C-terminal catalytic domain, a central dimerization domain, and an N-terminal regulatory domain. The central dimerization domain of the β1 subunit, which contains an N-terminal binding site (NBS) and a C-terminal binding site (CBS), has been postulated to be responsible for the formation of α/ β heterodimer. In this study, we analyzed heterodimerization by the pull-down assay using the affinity between a histidine tag and Ni2+ Sepharose after co-expression of various N- and C-terminally truncated FLAG-tagged mutants of the α1 subunit and the histidine-tagged wild type of the β1 subunit in the vaculovirus/Sf9 system, and demonstrated that the CBS-like sequence of the α1 subunit is critical for the formation of the heterodimer with the β1 subunit and the NBS-like sequence of the α1 subunit is essential for the formation of the enzymatically active heterodimer, although this particular sequence was not involved in heterodimerization. The analysis of the secondary structure of the α1 subunit predicted the existence of an amphipathic α-helix in residues 431-464. Experiments with site-directed α1 subunit mutant proteins demonstrated that the amphipathicity of the α-helix is important for the formation of the heterodimer, and Leu463 in the α-helix region plays a critical role in the formation of a properly arranged active center in the dimer.
- 社団法人日本動物学会の論文
- 2005-07-25
著者
-
Suzuki Norio
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Shiga Takumi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
-
Shiga Takumi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
関連論文
- Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
- Role of Boundary Plasma in Lower-Hybrid-Frequency Heating of a Tokamak
- Diamagnetic Measurement of JFT-2 Plasma Heated by Neutral Beam Injection
- Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6).
- Enterotoxin/guanylin receptor type guanylyl cyclases in non-mammalian vertebrates
- Metal Impurity Reduction by Working Gas Injection in a Tokamak
- On the Origin of Arcing in a Tokamak
- Sequence Analysis of cDNA and Genomic DNA, and mRNA Expression of the Medaka Fish Homolog of Mammalian Guanylyl Cyclase C
- Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae
- EXPRESSION OF AD4BP/SF-1 IN RED-SPOTED DWARF GOBY, TRIMMA OKINAWAE, DURING MULTI-SEX CHANGE(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- TWO TYPES OF P450 AROMATASE DURING SERIAL ADULT SEX CHANGE IN GOBIID FISH Trimma okinawae : cDNA ISOLATION AND mRNA EXPRESSION
- Diverse forms of guanylyl cyclases in medaka fish -- their genomic structure and phylogenetic relationships to those in vertebrates and invertebrates.
- Experimental Study on Limit of the Safety Factor in JFT-2 Tokamak
- Expression and Genomic Organization of A Medaka Fish Novel Membrane Form of Guanylyl Cyclase/Orphan Receptor
- Localization of the Nitric Oxide/cGMP Signaling Pathway-Related Genes and Influences of Morpholino Knock-Down of Soluble Guanylyl Cyclase on Medaka Fish Embryogenesis
- Expression of Membrane-Bound and Soluble Guanylyl Cyclase mRNAs in Embryonic and Adult Retina of the Medaka Fish Oryzias latipes
- Mass Spectrometric Analysis of Phosphoserine Residues Conserved in the Catalytic Domain of Membrane-Bound Guanylyl Cyclase from the Sea Urchin Spermatozoa
- A mRNA for Membrane Form of Guanylyl Cyclase Is Expressed Exclusively in the Testis of the Sea Urchin Hemicentrotus pulcherrimus
- EXPRESSION OF PARTICULATE AND SOLUBLE GUANYLYL CYCLASE mRNA IN THE MEDAKA RETINA(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Molecular Phylogenetic Analysis of Diverse Forms of Echinoderm Guanylyl Cyclases(Biochemistry)
- Expression of Sperm-Activating Peptide IV Receptor-Associated Membrane Guanylyl Cyclase in the Testis of the Sea Urchin Diadema setosum
- Expression of Sperm-Activating Peptide IV Receptor-Associated Membrane Guanylyl Cyclase in the Testis of the Sea Urchin Diadema setosum
- Primary structure of a 120kDa protein associated with the fucose sulfate glycoconjugate constituting the acrosome reaction-inducing substance of the sea urchin, Hemicentrotus pulcherrimus
- Electron Heat Conduction Coefficients at the Temperature Rise Phase and the Internal Disruption during the Sawtooth Oscillation of Tokamak
- The Guanylyl Cyclase Family in Medaka Fish Oryzias latipes
- Expression and Exon/Intron Organization of Two Medaka Fish Homologs of the Mammalian Guanylyl Cyclase A^1
- Characterization of Sea Urchin Sperm Membrane Proteins which Interact with a Major Acrosome Reaction-Inducing Substance, Fucose Sulfate Glycoconjugate
- Identification of tandem organization of soluble guanylyl cyclase α_1 and β_1 subunit genes in the Japanese pufferfish (Fugu rubripes) genome: comparison with their human homologues
- Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene
- Dephosphorylation of Autophosphorylated Regulatory Subunit of Sea Urchin Sperm cAMP-Dependent Histone Kinase by an Endogenous Protein Phosphatase
- Genomic Structure and Expression of the Medaka Fish Homolog of the Mammalian Guanylyl Cyclase B^1
- SPERM-ACTIVATING PEPTIDES IN THE EGG JELLY OF SEA URCHINS (Abstracts of papers read by the Zoological Society Prize winners)
- Amphipathic alpha-helix mediates the heterodimerization of soluble guanylyl cyclase.