Establishment and Characterization of Rat Dental Epithelial Derived Ameloblast-Lineage Clones(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Teeth are the hardest tissues covered with enamel produced by ameloblasts. The ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions during tooth morphogenesis. However, the molecular mechanism of ameloblast differentiation remains unclear. To address this question, we developed an in vitro assay system to evaluate the molecular mechanism of amelogenesis. First, we established dental epithelium-derived clones from 6-day-old rat incisors and established that cells of the clone SRE-G5 were the largest producers of amelogenin mRNA. Next, we analyzed the effects of several chemicals on the amelogenin expression in SRE-G5 cells. Only mitogen-activated protein kinase (MAPK) activators enhanced amelogenin mRNA expression. This finding corresponded to the immunohistochemical data showing the presence of phosphorylated forms of p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) during ameloblast differentiation. To examine the roles of MAPK signals, we compared the effects of anisomycin and sodium salicylate on the expression of tooth-related differentiation markers. Both anisomycin and sodium salicylate induced amelogenin, Abcg2, and Bmp4 mRNA and down-regulated p75NGFR mRNA. On the other hand, ALP, ectodin, Bmp2 and Fgf8 mRNA were up-regulated only by anisomycin. These results indicate that MAPK signaling functions, at least in part, as the inducer of ameloblast differentiation.
- 社団法人日本生物工学会の論文
- 2007-05-25
著者
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Miyoshi Keiko
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Horiguchi Taigo
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Noma Takafumi
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Muto Taro
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Noma Takafumi
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Noma Takafumi
徳島大学 ヘルスバイオサイエンス研究部分子医科学
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Muto Taro
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Miyoshi Keiko
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Miyoshi Keiko
Department Of Biochemistry School Of Dentistry The University Of Tokushima
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Abe Kaori
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Ruspita Intan
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Nagata Toshihiko
Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of T
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Horiguchi Taigo
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Abe Kaori
Department Of Internal Medicine And Rheumatolory Juntendo University School Of Medicine
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Abe Kaori
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Ruspita Intan
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Nagata Toshihiko
Department Of Periodontology And Endodontology Institute Of Health Biosciences The University Of Tok
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Nagata Toshihiko
Department Of Neuropsychiatry Osaka City University Medical School
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ABE Kaori
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine
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