Generation of human induced pluripotent stem cells from oral mucosa(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
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Induced pluripotent stem (iPS) cells are one of the most promising sources for cell therapy in regenerative medicine. Using a patient's own genetically identical and histocompatible cells is the ideal way to practice personalized regenerative medicine. For personalized iPS cell therapy, the prerequisites for cell source preparation are a simple and safe procedure, no aesthetic or functional damage, and quick wound healing. Oral mucosa fibroblasts (OFs) may have high potential to fulfill these requirements. In this study, biopsy was performed in a dental chair; no significant incisional damage was recognized and rapid wound healing (within a week) was observed. We generated human iPS cells from the isolated OFs via the retroviral gene transfer of OCT4, SOX2, c-MYC, and KLF4. Reprogrammed cells showed ES-like morphology and expressed undifferentiated markers such as OCT4, NANOG, SSEA4, TRA-1-60, and TRA-1-81. Subsequent in vitro and in vivo analyses confirmed the pluripotency of resultant iPS cells, which matched the criteria for iPS cells. In addition, we found that the endogenous expression levels of c-MYC and KLF4 in OFs were similar to those in dermal fibroblasts. Taken together, we propose that OFs could be a practical source for preparing iPS cells to achieve personalized regenerative medicine in the near future.
著者
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Miyoshi Keiko
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Noma Takafumi
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Tsuji Daisuke
Department of Medicinal Biotechnology, Institute of Health Biosciences, The University of Tokushima
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Kudoh Keiko
Department of Oral and Maxillofacial Surgery, Institute of Health Biosciences, The University of Tok
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Satomura Kazuhito
Department of Oral and Maxillofacial Surgery, Institute of Health Biosciences, The University of Tok
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Muto Taro
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Gradua
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Itoh Kohji
Department of Medicinal Biotechnology, Institute of Health Biosciences, The University of Tokushima
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Kudoh Keiko
Department Of Cardiovascular Diseases Fukuoka University Chikushi Hospital
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Noma Takafumi
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Noma Takafumi
徳島大学 ヘルスバイオサイエンス研究部分子医科学
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Muto Taro
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Itoh Kohji
Department Of Medicinal Biotechnology Institute Of Health Biosciences The University Of Tokushima Gr
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Kudoh Keiko
Department Of Oral And Maxillofacial Surgery Institute Of Health Biosciences The University Of Tokus
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Tsuji Daisuke
Department Of Medicinal Biotechnology Institute For Medicinal Resources Graduate School Of Pharmaceu
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Tsuji Daisuke
Department Of Medicinal Biotechnology Institute Of Health Biosciences The University Of Tokushima Gr
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Miyoshi Keiko
Department Of Molecular Biology Institute Of Health Biosciences The University Of Tokushima Graduate
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Miyoshi Keiko
Department Of Biochemistry School Of Dentistry The University Of Tokushima
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Itoh Kohji
Department Of Biology Chiba University
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Satomura Kazuhito
Department Of Oral And Maxillofacial Surgery Institute Of Health Biosciences The University Of Tokus
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Itoh Kohji
Department Of Applied Electronics Science University Of Tokyo
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