Effect of Calcium Phosphate Cement Implanted in Alveolar Bone Defects in Dogs : Appearance of Bone Sialoprotein at the Early Stages of Bone Regeneration
スポンサーリンク
概要
- 論文の詳細を見る
The effect of a calcium phosphate cement (CPC) on bone regeneration was investigated by implanting CPC particles into alveolar bone defects formed in adult dogs aud examining the histological and biochemical chenges in the periodontal tissues surrounding the defects. Hydroxyapatite (HAP)- and non-implanted control groups were also prepared and compared with the CPC group. Histological observations 2 weeks post-implantation revealed that CPC was rapidly absorbed, probably by osteoclast-like cells, which showed positive tartrate-resistant acid phosphatase (TRAP) staining, and a high proportion of the defect was filled with fibrous tissue. After 24 weeks, the fibrous tlssue in the CPC group had been replaced with mature bone-like tissue. Although bone formation was also observed in the HAP and control groups, the greatest amount of newly formed bone was observed in the CPC group after 24 weeks. The TRAP and alkaline phosphatase (ALP) activities of fibrous tissues from around the defects of the three groups 1, 2, and 3 weeks post-implantation were determined and compared. The 2-week samples showed that the TRAP activity in the CPC group was about twice that in the HAP group, indicating high osteoclastic activity in the former. Furthermore, the ALP activity in the CPC group increased markedly by 2.8- 4.0-fold the HAP and control group values after 1-3 weeks. Western blotting analysis revealed that 68 kDa bone sialoprotein (BSP), which is important in the initial stage of bone formation, was present in the CPC group tissues after 1-3 weeks, indicating incrcased osteoblastic activity. These findings suggest that active bone remodeling was induced through a coupling phenomenon by CPC-absorption and then the bone mass increased in association with osteoblastic BSP production during the early stages of bone regeneration.
- 硬組織再生生物学会の論文
著者
-
Nagata Toshihiko
Institute of Health Biosciences
-
ISHIDA Hiroshi
Department of Periodontology and Endodontology, Tokushima University School of Dentistry
-
Iwamoto Osamu
Tokuyama Co. Ltd.
-
Nishikawa Seiji
Department Of Periodontology And Endodontology Tokushima University School Of Dentistry
-
Nagata Toshihiko
Department Of Periodontology And Endodontology Institute Of Health Bioscience The University Of Toku
-
Nagata Toshihiko
Department Of Neuropsychiatry Osaka City University Medical School
-
NISHIMURA Yasuyoshi
Department of Periodontology and Endodontology, Tokushima University School of Dentistry
-
Nishimura Yasuyoshi
Department Of Periodontology And Endodontology Tokushima University School Of Dentistry
-
Ishida Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
関連論文
- 2P-225 プロテオミクス解析により明らかにされたミトコンドリア膜の透過性に対するCa^とバリノマイシンの作用の違い(細胞生物学的課題(2),第46回日本生物物理学会年会)
- Establishment and Characterization of Rat Dental Epithelial Derived Ameloblast-Lineage Clones(CELL AND TISSUE ENGINEERING)
- High-turnover osteoporosis is induced by cyclosporin A in rats
- Drug-Induced Gingival Overgrowth : a Review
- Regulation of tenascin expression in cultured rat dental pulp cells
- Relationship between the expression of parathyroid hormone receptors and hormonal effect during rat osteoprogenitor cell differentiation
- Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus
- Fluorescence Resonance Energy Transfer between Points on Tropomyosin and Actin in Skeletal Muscle Thin Filaments: Does Tropomyosin Move?^1
- ACCELERATION OF MANGANESE UPTAKE BY RICE PLANT GROWN UNDER UNFAVORABLE TEMPERATURE OR LIGHT CONDITION
- Bisphosphonate-related osteonecrosis of the jaw : position paper from the Allied Task Force Committee of Japanese Society for Bone and Mineral Research, Japan Osteoporosis Society, Japanese Society of Periodontology, Japanese Society for Oral and Maxillof
- Effects of TGF-β1 on Extracellular Matrix Formation in Rat Clonal Dental Pulp Cells
- Visual and auditory hallucinations with excessive intake of paroxetine
- Single and married patients with eating disorders
- Outcome of severe anorexia nervosa patients receiving inpatient treatment in Japan : An 8-year follow-up study
- Comparison between sevoflurane and isoflurane anesthesia in pig hepatic ischemia-reperfusion injury
- Are young women in Japan at high risk for eating disorders? : Decreased BMI in young females from 1960 to 1995
- Reliability and validity of the Japanese version of the Yale-Brown Obsessive-Compulsive Scale
- Physical and sexual abuse histories in patients with eating disorders : A comparison of Japanese and American patients
- Reassociation of the Small Form of Ferredoxin-NADP^+ Reductase to the Large Form
- A New Protein Factor, Connectein, as a Constituent of the Large Form of Ferredoxin-NADP Reductase
- Preparation and Properties of PbTiO_3 Films by Sol-Get Processing
- Dissection of the effects of tumor necrosis factor-α and class II gene polymorphisms within the MHC on murine systemic lupus erythematosus(SLE)
- Prostaglandin E_2 Inhibits Alveolar Bone Resorption in Experimental Periodontitis in Hamsters
- Effect of Calcium Phosphate Cement Implanted in Alveolar Bone Defects in Dogs : Appearance of Bone Sialoprotein at the Early Stages of Bone Regeneration
- Influence of Active Fingertip Contact with a Stable Surface on Postural Sway and Electromyographic Activities of the Lower Extremity Muscles Immediately after Descending a Step
- Influence of Neck Flexion on Lumbar Curvature during Bridging Exercises
- An Electromyographic Analysis of Trunk and Hip Extensor Muscles during Bridging Exercises : Effect of Voluntary Control of the Pelvic Tilt
- Sensor Systems for Detecting Gas Plumes-Robots and Sensor Network (特集:燃料電池を支えるMEMS/NEMS技術)
- Influence of Active Fingertip Contact with a Stable Surface on Postural Sway and Electromyographic Activities of the Lower Extremity Muscles Immediately after Turning