89.STUDIES ON THE DIFFERENTIATION FROM THE CALLUS OF CICHORIEAE SPECIES

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From the leaflet segment of Sonchus oleraceus cultured on K medium, the callus formed smoothly. Calli developed well on K medium, and they differentiate into various forms and colors. The authors observed them through a stereo-microscope, and some of the photographs are included the text. The calli consisted of light-green or white colored primordia mass, and developed root-like shoot structures. Epidermal tissue developed in white dome-like callus, in which tracheid cells or tissue developed. After a while somewhat long roots sprouted from it. The callus of Lactuca indica were apt to become brown-colored from the effect of the secretion of latex from the cut surface of the leaflet. We obtained the callus of this species for the first time using for the material a young leaf segment on K medium. Thereafter the callus grew well on K medium and granular primordia came out successively. White, light green, reddish purple cell clump were there, among them green dome-shape calli were conspicuous. These green dome-shaped calli consisted of many mitosis cells and the morphology of the cells were diverse. The leaflet or buds primordia developed and grew root-like shoot structures later on.
1990-03-15