ギボウシの発芽種子に誘導されたカルスからの再分化によるin vitro増殖

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キーワード：カルス，ギボウシ，再分化，組織培養，増殖　When seeds of Hosts sieboldiana (Lodd.) Engl. were germinated and then cultured on AZ medium containing 10μM 2.4- diclorophenoxyacetic acid and 0.3 to 1μM 6-(N-benzylamino) purine (BA), the highest frequency of callus growth was induced. Hypocotyl of a germinating seed was the site of callus initiation. Culturing the callus on the AZ medium containing 3μM BA and 0.3 to 10μM 1 -naphthaleneacetic acid (NAA) induced the highest frequency of shoot initiation from callus. The AZ medium containing 0.3 to 10μM N^6 -(2 -isopentenyl) adenine (or 0.3μM BA) and 0.3 to 10μM NAA induced the highest frequency of root initiation from callus. Almost all cultures with 3 or less shoots developed all the shoots after acclimatization, but many of cultures with 4 or more shoots withered some shoots during acclimatization and following cultivation. Immature flower buds and the tip of an immature inflorescence before bolting were efficient explant sources for in vitro propagation using callus induction.　Key words: callus. Hosta, propagation, regeneration, tissue culture,
山形大学の論文
2005-02-16