Decline in Glucokinase Activity in the Arcuate Nucleus of Streptozotocin-Induced Diabetic Rats(Biochemistry)
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概要
- 論文の詳細を見る
Glucokinase (GK) is known to be the critical glucose sensor of pancreatic B-cells. However, the localization and functional role of GK in the brain remains to be elucidated. In this study, we measured both the activity and mRNA level of GK in the hypothalamic nuclei and the cortex of rats injected intraperitoneally with streptozotocin or vehicle. GK activity was measured by a fluorometric assay; and the GK mRNA level, by use of the real-time reverse transcription polymerase chain reaction. GK activity in vehicle-treated rats was high in the arcuate nucleus, moderate or low in the ventromedial nucleus, lateral hypothalamic area, and paraventricular nucleus, and very low in the cortex. The order of GK mRNA level was almost the same as that of GK activity. GK activity and GK mRNA level only in the arcuate nucleus of streptozotocin-treated rats at 7d, but not at 2d, after treatment were lower than those of vehicle-treated rats. The results suggest that prolonged hyperglycemia induced by diabetes decreased the activity of GK in the arcuate nucleus.
- 公益社団法人日本薬学会の論文
- 2006-02-01
著者
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MIWA Ichitomo
Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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TOYODA Yukiyasu
Department of Pathobiochemisty, Faculty of Pharmacy, Meijo University
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CHIBA Taku
Faculty of Pharmaceutical Sciences, Nagoya City University
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Chiba Taku
Faculty Of Pharmacy Kinjo Gakuin University
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Chiba Taku
Faculty Of Pharmaceutical Sciences Nagoya City University
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Nishio Takashi
Laboratory Of Neuropsychopharmacology Graduate School Of Environmental And Human Sciences Meijo Univ
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Toyoda Yukiyasu
Department Of Pathobiochemistry Faculty Of Pharmacy Meijo University
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HIRAMATSU Masayuki
Laboratory of Neuropsychopharmacology, Graduate School of Environmental and Human Sciences, Meijo Un
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Miwa Ichitomo
Department Of Pathobiochemistry Faculty Of Pharmacy Meijo University
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Hiramatsu Masayuki
Laboratory Of Neuropsychopharmacology Graduate School Of Environmental And Human Sciences Meijo Univ
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MIWA ICHITOMO
Department of Clinical Biochemistry, Faculty of Pharmaceutical Science, Meijo University
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