Segmental Heterogeneity of Epithelial Ion Transport Induced by Stimulants in Rat Distal Colon(Molecular and Cell Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Mammalian colon plays an important role in electrolyte and water balance, and exhibits significant segmental heterogeneity. The different responses to stimulants even exist in different segments of rat distal colon. The present study focus on the segmental heterogeneity of epithelial responses to different stimulants and investigates the possible mechanism by using short circuit current recording technique. Baseline I_<sc> in the segment 4 of distal colon (DC_4, 20.8±2.8μA・cm^<-2>) was significantly lower than that in the segment 1 of distal colon (DC_1, 40.5±1.9μA・cm^<-2>). Basolateral application of indomethacin induced a larger reduction of the baseline I_<sc> in DC_4 (-28.2±3.9μA・cm^<-2>) than that in DC_1 (-10.1±3.9μA・cm^<-2>). Moreover DC_4 are more sensitive to foskolin (a cAMP activator, apical side), Ach (basolateral side) and 5-HT (basolateral side) than DC_1, which was not affected by pretreatment with amiloride, a blocker of epithelial Na^+ channel. Basolateral pretreatment with atropine (muscarinic cholinergic receptor antagonist) for 10 min, Ach-induced ΔI_<SC> increases in both DC_1 and DC_4 were totally blocked. Otherwise, 5-HT_4 receptor antagonist GR113808 (basolateral side) and SB-204070 (basolateral side) completely inhibited 5-HT-induced I_<SC> increases in both DC_1 and DC_4. Taken together, the results suggested that the segmental heterogeneity of epithelial responses to different stimulants exists in rat distal colon. And it is more likely related to the dissimilar distribution of membrane proteins involved in the ion transports within different segments of rat distal colon.
- 公益社団法人日本薬学会の論文
- 2006-09-01
著者
-
Chen Xin
Department of Cardiology, National Cardiovascular Center
-
Chen X
Faculty Of Life And Environmental Science Shimane University
-
Chen X
Department Of Radiochemistry-biophysics And Functional Food Sciences Niigata University Of Pharmacy
-
Zhu J
Epithelial Cell Biology Research Center Department Of Physiology Faculty Of Medicine The Chinese Uni
-
Zhu Jin-xia
Department Of Physiology Basic Medical College Capital Medical University
-
Chen Xin
Department Of Physiology Basic Medical College Capital Medical University
-
Chen Xin
Department Of Cardiology National Cardiovascular Center
-
XUE Hong
Department of Physiology, Basic Medical College, Capital Medical University
-
YANG Ning
Department of Physiology, Basic Medical College, Capital Medical University
-
GUO Hua
Department of Physiology, Basic Medical College, Capital University of Medical Sciences
-
Yang Ning
Department Of Physiology Basic Medical College Capital Medical University
-
Guo Hua
Department Of Cardiology Clinical School Of Medical College Nanjing University
-
Xue Hong
Department Of Physiology Basic Medical College Capital Medical University
-
Xue Hong
Department Of Civil Engineering National University Of Singapore
-
Guo Hua
Department Of Physiology Basic Medical College Capital University Of Medical Sciences
関連論文
- Effect of Systolic Blood Pressure on Transmural Myocardial Strain Profile(Echo/Doppler 2 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Transmural Myocardial Strain Gradient : A New and Robust Index for Quantifying Myocardial Regional Systolic Function(Echo/Doppler 6 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Novel Surgical Method of Proximal Anastomosis in Off-Pump Coronary Artery Bypass Grafting
- Radial Artery as Conduit is Safe and Effective in Coronary Bypass Surgery in the Elderly : Single-Central Results From 326 Patients
- Effect of Sodium Ferulate on Human Colonic Anion Secretion and the Underlying Signaling Mechanism(Pharmacology)
- Effect of Bak Foong Pills on Exocrine Pancreatic-Bile Secretion(Pharmacology)
- Cholesterol Biosynthesis Inhibitory Activities of Novel Triterpenoid Acids from Kadsura heteroclita and K. longipedunculata(Biological Chemistry)
- PC37 NOVEL TRITERPENOID ACIDS WITH INHIBITORY ACTIVITIES AGAINST CHOLESTEROL BIOSYNTHESIS FROM KADSURA HETEROCLITA AND K. LONGIPEDUNCULATA
- PA53 NEOKADSURANIC ACID A AND SECO-NEOKADSURANIC ACID A, THE FIRST TWO TRITERPENOIDS WITH 14 (13→12) ABEO LANOSTANE SKELETON FROM KADSURA HETEROCLITA
- Appearance of Segmental Discrepancy of Anion Transport in Rat Distal Colon(Molecular and Cell Biology)
- Segmental Heterogeneity of Epithelial Ion Transport Induced by Stimulants in Rat Distal Colon(Molecular and Cell Biology)
- PA70 NEOKADSURANIC ACID B AND C, NOVEL TRITERPENOID ACIDS WITH 14 (13→12) ABEO LANOSTANE SKELETON FROM KADSURA LONGIPEDUNCULATA
- Baicalin Promoted the Repair of DNA Single Strand Breakage Caused by H_2O_2 in Cultured NIH3T3 Fibroblasts
- 22-46 Removal of Colored Substances and COD in Livestock Wastewater by Soil-based Treatment Systems : The Effect of Ultraviolet Irradiation Pretreatment on the Organic Matter Decomposition in the Wasterwater
- Effect of aeration and material composition in soil mixture block on the removal of colored substances and chemical oxygen demand in livestock wastewater using multi-soil-layering systems(Environment)
- Effect of structural difference on wastewater treatment efficiency in multi-soil-layering systems : Relationship between soil mixture block size and removal efficiency of selected contaminants(Environment)
- Comparative study of soils and other adsorbents for decolorizing sewage and livestock wastewater(Environment)
- 22-13 Effect of Structure Difference on Wastewater Treatment Efficiency by Multi-soil-layering System
- Study of structure difference influence on wastewater treatment efficiency by Multi-soil-layering system
- Comparison of Myocardial Bridging by Dual-Source CT With Conventional Coronary Angiography
- Temperature Stratification of Heated Air Flow in a Fire Tunnel
- Involvment of Endogenous Prostaglandin in Emodin-Evoked Rat Colonic Anion Secretion(Pharmacology)
- Kinetic Energy Budgets of the Low-Level Jet during TAMEX IOP 5
- Profile measurement of a wide-area resist surface using a multi-ball cantilever system
- Localized persistent interstitial pulmonary emphysema presenting as a spontaneous tension pneumothorax in a full term infant
- Ge/Ni-InGaAs Solid-State Reaction for Contact Resistance Reduction on n In.Ga.As (Special Issue : Solid State Devices and Materials (1))