Involvment of Endogenous Prostaglandin in Emodin-Evoked Rat Colonic Anion Secretion(Pharmacology)
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概要
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It has been reported that emodin is able to promote gastrointestinal motility and stimulate large intestinal water secretion ; however, the mechanism is still not clear. The aim of the present study is to examine the effects of emodin on the rat colonic transepithelial ion transport and the underlying mechanism. The study was carried out by means of the short circuit current (I_<SC>) recording. Basolateral application of emodin induced a concentration-dependent I_<SC> increase, and the EC_<50> was 76.0μmol/l. Pretreatment with epithelial Na^+ channel blocker, amiloride (10μmol/l), did not affect the I_<SC> responses elicited by emodin, but removal of extracellular Cl^- or apical pretreatment with Cl^- channel blocker, glibenclamide (1 mmol/l) inhibited emodin-elicited I_<SC> responses by 76.3% and 83.8% respectively. Inhibiting basolateral Na^+-K^+-2Cl^- cotransporter (NKCC) with bumetanide (100μmol/l) decreased emodin-induced I_<SC> from 118.1±6.7, μA/cm^2 to 16.7±2.0μA/cm^2, which was reduced by 85.9%. Basolateral pretreatment with neuronal Na^+ channel blocker tetrodotoxin (TTX) (1μmol/l) did not affect emodin-induced I_<SC> increase, but pretreatment with indomethacin (10μmol/l) alone or with both TTX and indomethacin significantly decreased emodin-induced I_<SC> increase by 88.4 and 81.2%, respectively. The present study demonstrated that emodin was able to stimulate rat colonic epithelial Cl^- secretion, which was predominantly mediated by endogenous prostaglandin release.
- 公益社団法人日本薬学会の論文
- 2007-11-01
著者
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Chen Xin
Department of Cardiology, National Cardiovascular Center
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Zhu Jin-xia
Department Of Physiology Basic Medical College Capital Medical University
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Xu Jing-dong
Department Of Physiology Basic Medical College Capital Medical University
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Chen Xin
Department Of Physiology Basic Medical College Capital Medical University
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Chen Xin
Department Of Cardiology National Cardiovascular Center
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Wang Wen
Institute Of Electro-optical Engineering National Taipei University Of Technology
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LI Li-Sheng
Department of Physiology, Basic Medical College, Capital Medical University
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Li Li-sheng
Department Of Physiology Basic Medical College Capital Medical University
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Wang Wen
Institute Of Acoustics Chinese Academy Of Sciences
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Wang Wen
Institute Of Pharmacology Xuanwu Hospital Capital Medical University
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