Appearance of Segmental Discrepancy of Anion Transport in Rat Distal Colon(Molecular and Cell Biology)
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概要
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The present study investigated the segmental discrepancy of the rat distal colonic anion transport induced by luminal forskolin and the possible underlying mechanisms using short-circuit current recording technique and comparative quantity real-time PCR analysis. Forskolin-induced I_<SC> in the segment next to lymph node (DC_1) and the segment 4cm away from lymph node (DC_4) were 4.09±0.66 μA/cm^2 and 18.84±3.18 μA/cm^2 (n=13), respectively, which were blocked by luminal Cl^- channel blocker, glybenclamide (1 mM) (n=5,p<0.01), as well as removal of extracellular Cl^- and HCO^-_3 in both DC_1 and DC_4 (n=5, p<0.001). Furthermore luminal pretreatment with K^+ blockers, TEA (5 mM) and glybenclamide (100 μM) didn't affect forskolin and bumetanideenhanced I_<sc>. Reducing serosal Cl^- concentration increased forskolin-induced I_<sc> by 90% in DC_1 but decreased forskolin-induced I_<sc> in DC_4 by 50%. Furthermore, pretreatment with serosal bumetanide, an inhibitor of Na^+_- K^+-2Cl^- cotransporter, enhanced forskolin-induced I_<sc> by 87% in DC_1, from 4.09±0.66 μA/cm^2 to 7.65±0.53 μA/c^m2 (n=6, p<0.01), but inhibited forskolin-induced I_<sc> by 50% in DC_4, from 29.19±4.51 μA/cm^2 to 15.06±4.10 μA/cm^2 (n=6, p<0.05). Pretreatment with luminal amiloride (10 μM), an inhibitor of ENaC, and serosal 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) (200 μM), an inhibitor of NBC, significantly inhibited the forskolin-induced I_<sc> in DC_1 (n=6, p<0.05), but not in DC_4. Real-time PCR analysis did not show the significant differences between the two segments in the expression amounts of CFTR and NKCC mRNAs, however the expression of NBC mRNA in DC_4 was significantly higher than that in DC_1. In conclusion, the rat distal colon manifests a segmental discrepancy in anion transport stimulated by luminal forskolin. HCO^-_3 might be predominantly involved in the forskolin-induced anion secretion in DC_1, but Cl^- might be the main component of the anion secretion in DC_4.
- 公益社団法人日本薬学会の論文
- 2007-08-01
著者
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Chen Xin
Department of Cardiology, National Cardiovascular Center
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Tian Yue
Department Of Physiology Basic Medical College Capital Medical University
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Tian Yue
中華人民共和国
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Chen X
Faculty Of Life And Environmental Science Shimane University
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Chen X
Department Of Radiochemistry-biophysics And Functional Food Sciences Niigata University Of Pharmacy
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Zhu J
Epithelial Cell Biology Research Center Department Of Physiology Faculty Of Medicine The Chinese Uni
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XING Ying
Departments of Physiology and Pathophysiology, Medical School, Zhengzhou University
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Yan Ming
Department Of Physiology Medical School Zhengzhou University
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Chen Xin
Department Of Physiology Basic Medical College Capital Medical University
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Chen Xin
Department Of Cardiology National Cardiovascular Center
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XUE Hong
Department of Physiology, Basic Medical College, Capital Medical University
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YANG Ning
Department of Physiology, Basic Medical College, Capital Medical University
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ZHU Jin
Department of Physiology, Basic Medical College, Capital Medical University
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Yang Ning
Department Of Physiology Basic Medical College Capital Medical University
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Xue Hong
Department Of Physiology Basic Medical College Capital Medical University
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Xue Hong
Department Of Civil Engineering National University Of Singapore
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Xing Ying
Department Of Physiology Basic Medical College Zheng Zhou University
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Yan Ming
Department Of Orthopaedic Peking University Third Hospital
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Zhu Jin
Department Of Physiology Basic Medical College Capital Medical University
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Yan Ming
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University
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