Effect of Sodium Ferulate on Human Colonic Anion Secretion and the Underlying Signaling Mechanism(Pharmacology)

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The present study investigated the effect of ferulic acid, a compound purified from traditional Chinese herbal medicine Chuanxiong and Awei, on anion secretion by human colonic cells (T_<84>) using the short circuit current (I_<SC>) and microspectrofluorimetric technique. Basolateral administration of sodium ferulate (SF) produced a concentration-dependent increase of I_<SC> in T_<84> cells with an EC_<50> of 1.2mM. The SF-induced increase in I_<SC> contained a transient peak followed by a sustained plateau. Removal of extracellular Cl^-, basolateral addition of bumetanide, an inhibitor of the Na^+-K^+-Cl^- cotransporter (NKCC) and apical pretreatment with DPC, a Cl^- channels blocker, decreased the SF-induced increase in I_<SC> by 94% (p<0.001), 84% (p<0.001) and 85% (p<0.001) respectively. Pretreatment with thapsigargin, a specific microsomal Ca^<2+>-ATPase inhibitor, in combination with EGTA, a Ca^<2+> chelator, decreased the SF-induced peak by 52% (p<0.01) and inhibited the SF-induced plateau by 60% (p<0.05). Pretreatment with MDL12330A, an adenylate cyclase inhibitor, blocked the SF-induced I_<SC> plateau by 87% (p<0.01) but did not affect the SF-induced I_<SC> peak. Microspectrofluorimetric measurements show that SF induced a sustained increase in [Ca^<2+>]_i. The results suggested that SF could induce Cl^- secretion in T_<84> cells via Ca^<2+> and cAMP-dependent pathways.
社団法人日本薬学会の論文
2005-09-01