Topical Application of γ-Tocopherol Derivative Prevents UV-Induced Skin Pigmentation(Pharmacology)

概要

論文の詳細を見る
We previously reported that a novel hydrophilic γ-tocopherol (γ-Toc) derivative, γ-tocopheryl-N,N-dimethylglycinate hydrochloride (γ-TDMG) gets converted to the antioxidant γ-Toc in skin. We also found that this derivative displayed greater bioavailability than γ-Toc itself. In the present study, we determined whether γ-TDMG could reduce UV-induced skin pigmentation in brownish guinea pigs. γ-TDMG (0.1 or 0.5%) was topically applied to the skin before and after it was exposed to UVB plus UVA (3 times/week for 1 week), and then 10 times/week for 4 weeks thereafter. Treatment with 0.5% γ-TDMG resulted in significant skin lightening (70% of the pigmentation of irradiated controls). We also found that melanin synthesis was dose-dependently inhibited by γ-TDMG in murine B16 melanoma cells. When γ-TDMG or kojic acid (250μM) were added to homogenates of B16 melanoma cells, their tyrosinase activity was significantly inhibited by approximately 40% and 75%, respectively. Mushroom tyrosinase activity was significantly inhibited by 200μM γ-Toc and kojic acid, but not γ-TDMG. When B16 cells were incubated with 250μM γ-TDMG for 24 or 48h, their intracellular γ-Toc concentrations rose over 100 fold to 10.5 and 11.2 nmol/10^6 cells, respectively, suggesting that γ-TDMG was rapidly converted to γ-Toc in these cells and that their reduced melanin synthesis may have been due to the activity of γ-Toc. Our data further suggest that the topical application of γ-TDMG may be efficacious in preventing photo-induced skin pigmentation in humans.
公益社団法人日本薬学会の論文
2006-06-01

Topical Application of γ-Tocopherol Derivative Prevents UV-Induced Skin Pigmentation(Pharmacology)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク