Development and Characterization of Microsatellite Markers for Common Buckwheat

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概要

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• So far, only five microsatellite markers have been developed in common buckwheat (Fagopyrum esculentum). The purpose of the present study was to develop a larger number of microsatellite markers in common buckwheat. By sequencing 2785 clones from the libraries, which were enriched for (CT)_n and (GT)_n repeats using magnetic particles, it was shown that 1483 clones contained microsatellites, of which 352 had unique sequences. Primer pairs were designed for 237 of the microsatellite loci, of which 180 primer pairs each amplified PCR products. Fifty-four primer pairs that each amplified a clear PCR product of the expected size were evaluated for their ability to detect variations in common buckwheat populations and to be utilized in seven related Fagopyrum species. Forty-eight (88.9%) out of the 54 microsatellite markers tested were found to be highly variable (the average number of alleles was 12.2 and the average polymorphism information content (PIC) was 0.79) in a population of cultivated buckwheat. This PIC value was comparatively large when compared with the average values for microsatellite markers reported for other crops. A high rate of successful amplification of common buckwheat microsatellite markers was observed in closely related species; in particular all the 54 loci were successfully amplified in the wild ancestor of cultivated common buckwheat. The microsatellite markers developed in the present study should contribute to the promotion of molecular breeding in common buckwheat.

• 日本育種学会の論文

著者

• Tsumura Yoshihiko

  Department Of Forest Genetics Forestry And Forest Products Research Institute

• Tsumura Yoshihiko

  Forestry And Forest Products Res. Inst. Ibaraki Jpn

• 津村 義彦

  農林水産省林野庁森林総合研究所

• Iwata Hiroyoshi

  Department Of Information Science And Technology National Agricultural Research Center

• Ohnishi Ohmi

  Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University

• Tsumura Yoshihiko

  Tree Genetics Laboratory Department Of Forest Genetics Forestry And Forest Products Research Institu

• Ohsawa Ryo

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Iwata Hiroyoshi

  National Agricultural Res. Center Ibaraki Jpn

• Iwata Hiroyoshi

  Department Of Forest Genetics Forestry And Forest Products Research Institute

• Konishi Takehiko

  Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University

• Yashiro Kazutoshi

  Plant Biotechnology Institute, Ibaraki Agriculture Center

• Yasui Yasuo

  Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University

• Tsumura Yoshihiko

  Genome Analysis Laboratory Department Of Forest Genetics Forestry And Forest Products Research Insti

• Ohsawa Ryo

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Yasui Yasuo

  Laboratory Of Crop Evolution Graduate School Of Agriculture Kyoto University

• Tomita Keno

  Plant Biotechnology Institute Ibaraki Agriculture Center

• Ohnishi Ohmi

  Laboratory Of Crop Evolution Graduate School Of Agriculture Kyoto University

• Ohnishi Ohmi

  Lab. Of Crop Evolution Graduate School Of Agriculture Kyoto Univ.

• Ohsawa Ryo

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Konishi Takehiko

  Laboratory Of Crop Evolution Graduate School Of Agriculture Kyoto University

• Yashiro Kazutoshi

  Plant Biotechnology Institute Ibaraki Agriculture Center

• Tsuda Yoshiaki

  Department Of Ecology And Genetics Evolutionary Biology Centre Uppsala University

• Tsumura Yoshihiko

  Department Of Evolutionary Functional Genomics Evolutionary Biology Centre Uppsala University

• 津村 義彦

  Forestry And Forest Products Research Institute

• Yasui Yasuo

  Lab. Of Crop Evolution Graduate School Of Agriculture Kyoto Univ.

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