Single-strand conformation polymorphism of sequence-tagged site markers based on partial sequences of cDNA clones in Cryptomeria japonica
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概要
- 論文の詳細を見る
Sixty-seven sequence-tagged site (STS) markers were identified from partial sequences of cDNA clones obtained from the inner bark of Cryptomeria japonica. Polymorphisms of the STSs were investigated for the parental clones of a mapping population, Haara and Kumotooshi, using both single-strand conformation polymorphism (SSCP) and sequencing analysis. Twenty-two STSs showed nucleotide differences between Haara and Kumotooshi, of which 19 STS differences were detectable under the electrophoresis conditions we used here. We also analyzed SSC-polymorphism in 10 additional clones derived from various Japanese regions to evaluate the usefulness of the STSs developed here among other populations of C. japonica. Twenty-five, about 40%, of the STSs showed polymorphism under selected electrophoresis conditions. The genotype segregation for 19 STSs was investigated among the Haara × Kumotooshi F1 population, and these STS markers were mapped on a linkage map. SSCP analysis of STSs was efficient in terms of cost and time, and it allows detection of a sufficiently high proportion of polymorphisms to provide a convenient means for mapping of expressed sequences on a linkage map and for studying various aspects of population genetics.
- 日本遺伝学会の論文
- 2002-08-25
著者
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Tsumura Yoshihiko
Department Of Forest Genetics Forestry And Forest Products Research Institute
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Tsumura Y
Forestry And Forest Products Res. Inst. Ibaraki Jpn
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Iwata Hiroyoshi
Department Of Information Science And Technology National Agricultural Research Center
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MATSUMOTO Asako
Department of Forest Genetics, Forestry and Forest Products Research Institute
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Iwata Hiroyoshi
Department Of Forest Genetics Forestry And Forest Products Research Institute
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Ujino-ihara Tokuko
Department Of Forest Genetics Forestry And Forest Products Research Institute
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MATUSMOTO Asako
Department of Forest Genetics, Forestry and Forest Products Research Institute
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YOSHIMURA Kensuke
Department of Forest Genetics, Forestry and Forest Products Research Institute
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Matusmoto Asako
Department Of Forest Genetics Forestry And Forest Products Research Institute
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Matsumoto Asako
Department Of Forest Genetics Forestry And Forest Products Research Institute
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Yoshimura Kensuke
Department Of Forest Genetics Forestry And Forest Products Research Institute
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Tsumura Yoshihiko
Department Of Evolutionary Functional Genomics Evolutionary Biology Centre Uppsala University
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