Malignant Transformation by a Human Activated ras Oncogene of a Revertant Cell Line Derived from BK virus-induced Hamster Tumor
スポンサーリンク
概要
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A revertant cell line (Vn-324R) derived from BK virus-induced hamster tumor was transformed with a recombinant plasmid containing an activated human c-Ha-ras oncogene. The transformed cells obtained had growth properties characteristic of fully transformed cells: high saturation-density, growth at a low serum concentration, colony formation in soft-agar medium, and tumorigenicity in hamsters. Southern blot analysis of the integrated oncogene revealed the presence of multiple copies of the c-Ha-ras gene in transformed cells. Vn-324R was also transformed with total DNA extracted from the primary transformants. The secondary transformants had growth properties similar to those of primary transformants and contained the integrated c-Ha-ras gene. These results indicate that the activated c-Ha-ras gene can induce a malignant transformation of Vn-324R cells. Since transformants derived from Vn-324R can be readily assayed for malignancy by tumor formation in hamsters, Vn-324R may be useful for analysis of activated human oncogenes for malignancy.
- 北里大学の論文
- 1987-12-31
著者
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YOGO YOSHIAKI
Department of Urology, Faculty of Medicine, The University of Tokyo
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YUASA Yasuhito
Department of Hygiene and Oncology, Tokyo Medical and Dental University School of Medicine
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Yogo Yoshiaki
Department Of Urology Faculty Of Medicine The University Of Tokyo
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Yogo Yoshiaki
Department Of Viral Infection The Institute Of Medical Science The University Of Tokyo
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Yuasa Yasuhito
Department Of Hygiene Gunma University School Of Medicine
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Yuasa Yasuhito
Department Of Hygiene And Oncology Tokyo Medical And Dental University School Of Medicine
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Hara Kazuya
Department of Microbiology, Kitasato University School of Hygienic Sciences
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Taguchi Fumiaki
Department of Microbiology, Kitasato University School of Hygienic Sciences
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Hara Kazuya
Department Of Microbiology Kitasato University School Of Hygienic Sciences
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Taguchi Fumiaki
Department Of Clinical Microbiology School Of Allied Health Sciences Kitasato University
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Taguchi Fumiaki
Department Of Microbiology Kitasato University School Of Hygienic Sciences
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Hara Kazuya
Department of Applied Electronics, Tokyo University of Science, Noda, Chiba 278-8510, Japan
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